铁线莲‘朱卡’组织培养技术及再生体系的建立  

Tissue culture and plant regeneration of Clematis ‘Julka’

作　　者：高燕[1] 莫建彬[1] 付艳茹 奉树成 GAO Yan;MO Jianbin;FU Yanru;FENG Shucheng(Shanghai Botanical Garden,Shanghai Engineering Research Center of Sustainable Plant Innovation,Shanghai 200231,China)

机构地区：[1]上海植物园,上海城市植物资源开发应用工程技术研究中心,上海200231

出　　处：《南京林业大学学报（自然科学版）》2021年第3期109-116,共8页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：上海市科技兴农引进消化吸收再创新项目(沪农科引字(2017)第1-3号)。

摘　　要：【目的】以铁线莲‘朱卡’(Clematis‘Julka’)的带芽茎段为起始材料,通过组织培养方法建立再生体系。【方法】利用腋芽诱导—不定芽增殖—生根和愈伤组织诱导—增殖—分化—生根两种途径,建立该品种的组织培养再生体系,形成铁线莲‘朱卡’完整植株。【结果】铁线莲‘朱卡’组织培养最适宜灭菌条件为质量分数10%次氯酸钠溶液灭菌12min;带芽茎段诱导腋芽最适培养基为MS+NAA 0.1 mg/L+6-BA 1.0 mg/L,不定芽增殖最适培养基为MS+IBA 0.20 mg/L+6-BA 1.0 mg/L+GA3 0.2 mg/L;带芽茎段诱导愈伤组织最适培养基为MS+NAA 0.05 mg/L+6-BA 2.0 mg/L,愈伤组织增殖最适培养基为MS+NAA 0.20 mg/L+6-BA 2.0 mg/L,愈伤组织分化最适培养基为MS+NAA 0.03 mg/L+6-BA 3.0 mg/L;最佳生根培养基为1/2MS+IBA 0.3 mg/L。【结论】首次用两种器官发生方法建立了稳定高效的铁线莲‘朱卡’再生体系。直接形成不定芽时增殖倍数可达5.52。诱导愈伤组织途径的器官发生中,愈伤组织分化率可达76.7%,分化不定芽平均数超过3。两种途径诱导生根率都在90%以上。【Objective】Stem segments having buds of Clematis ‘Julka’ were used as the explants to construct a regeneration system. 【Method】The system was established via two ways: one is initiates from induction of axillary buds, then proliferation of adventitious buds, and rooting induction;the other is from callus induction and proliferation to differentiation, final rooting. 【Result】The method for explants surface sterilization was incubated in 10%(mass fraction) sodium hypochlorite for 12 min. The medium for inducing axillary buds was MS+NAA 0.1 mg/L+6-BA 1.0 mg/L and for adventitious buds proliferation was MS+IBA 0.20 mg/L+6-BA 1.0 mg/L+GA3 0.2 mg/L. The medium for callus induction was MS+NAA 0.05 mg/L+6-BA 2.0 mg/L, for callus proliferation was MS+NAA 0.20 mg/L +6-BA 2.0 mg/L, and for callus differentiation was MS+ NAA 0.03 mg/L +6-BA 3.0 mg/L. The Optimal rooting induction medium was 1/2 MS+IBA 0.3 mg/L. 【Conclusion】This study established a stable and high-efficient regeneration system in vitro for Clematis ‘Julka’ by using two ways of organogenesis. The multiplication rate was 5.52 when shoot organogenesis were formed directly. In the organogenesis of callus induction pathway, the ratio of callus differentiation was 76.7% and the average number of differentiated adventitious buds was more than 3. The rates of plantlet rooting in two ways were more than 90%.

关 键 词：铁线莲‘朱卡’ 腋芽诱导 愈伤组织诱导 愈伤组织分化 再生体系 

分 类 号：S682.19[农业科学—观赏园艺]