具有癌症诊疗潜力的多功能核壳纳米粒  

Multifunctional core-shell nanoparticles for potential cancer theranostics

作　　者：宋雪[1] 吴静悦 张娟[1] 吉严蓉 景杰翔 柯亨特[1] SONG Xue;WU Jingyue;ZHANG Juan;JI Yanrong;JING Jiexiang;KE Hengte(College of Pharmaceutical Sciences,Soochow Universtiy,Suzhou 215123,China)

机构地区：[1]苏州大学药学院,江苏苏州215123

出　　处：《材料科学与工艺》2021年第3期57-63,共7页Materials Science and Technology

基　　金：国家自然科学基金资助项目(81871480);江苏省大学生创新创业训练计划(201910285136Y)。

摘　　要：采用乳化-溶剂挥发法制备了同时携载Mn_(3)O_(4)纳米粒和CuS白蛋白纳米粒的诊疗一体化多功能核壳纳米粒,可用于磁共振造影成像手段指引下的肿瘤光热消融,实现癌症的高效诊治。首先用高温热解法制备出粒径均一、分散性较好的Mn_(3)O_(4)纳米粒,并通过蛋白模板法制备CuS白蛋白纳米粒,之后采用可降解性聚乳酸-羟基乙酸共聚物(PLGA)作为载体,通过超声乳化法制备同时携载Mn_(3)O_(4)和CuS纳米粒的核壳纳米粒(Mn_(3)O_(4)/PLGA@CuS),并进行理化性质表征及诊疗一体化性能评价。结果表明:紫外-可见-近红外分光光度计测定结果显示该纳米粒被成功制备,其在近红外区具有较强吸收;核壳纳米粒的平均粒径为160 nm,粒径分布较为均匀;体外磁共振造影成像结果显示随着纳米粒中Mn离子浓度的升高,其造影增强效果也明显提升,显示其具有Mn离子浓度依赖的磁共振成像效果,纵向弛豫率为1.351 mM^(-1)·s^(-1);在785及980 nm激光辐照下,该核壳纳米粒均显示出良好的光热升温效果。成功制备了具有良好的磁共振造影成像性能和显著的光热升温效应的核壳纳米粒,有望应用于肿瘤的诊疗一体化。Theranostic core-shell nanoparticles are designed and constructed by incorporation of Mn_(3)O_(4) nanoparticles and CuS albumin nanoparticles into polymeric nanocarriers,in order to achieve contrast enhanced magnetic resonance imaging(MRI)guided photothermal tumor ablation.In this study,Mn_(3)O_(4) nanoparticles were prepared by thermal decomposition approach with uniform particle size and good dispersity,while CuS albumin nanoparticles were fabricated using protein template method.Biodegradable block copolymer poly(lactic-co-glycolic acid)was than applied as nanocarrier to load both Mn_(3)O_(4) and CuS nanoparticles by ultrasonication,resulting in theranostic core-shell nanoparticles(Mn_(3)O_(4)/PLGA@CuS).The physicochemical properties of the core-shell nanoparticles were characterized,and their diagnostic and therapeutic capabilities were also evaluated.The absorption spectra showed the successful fabrication of core-shell nanoparticles with both Mn_(3)O_(4) and CuS nanoparticle payloads.The size of core-shell nanoparticles was about 160 nm.In vitro MRI imaging results showed that the contrast enhancement was improved along with the increase of the Mn concentration in nanoparticles,indicating concentration-dependent MRI contrast enhancement property with longitudinal relaxation rate(r1)of 1.351 mM^(-1)·s^(-1).The core-shell nanoparticles were demonstrated to have great photothermal effect under both 785 and 980 nm irradiation.Thereby,theranostic core-shell nanoparticles incorporated with both Mn_(3)O_(4) and CuS nanoparticles were successfully constructed,which have significant photo-induced thermal effect with near infrared laser irradiation,and good performance in MRI,indicating great potential for tumor theranostics.

关 键 词：诊疗一体化 核壳纳米粒 白蛋白 CUS Mn_(3)O_(4) 

分 类 号：R318.08[医药卫生—生物医学工程] TB324[医药卫生—基础医学]