绿豆晕疫病病原菌的分离和鉴定  

Isolation and identification of the pathogen of mung bean halo blight

作　　者：田晓燕 孔庆全 贺小勇 赵存虎 陈文晋 马显民 TIAN Xiaoyan;KONG Qingquan;HE Xiaoyong;ZHAO Cunhu;CHEN Wenjin;MA Xianmin(Institute of Plant Protection,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;Agriculture and Animal Husbandry Bureau of Ongniud Banner of Chifeng City,Ongniud Banner 024500,China)

机构地区：[1]内蒙古自治区农牧业科学院植物保护研究所,内蒙古呼和浩特010031 [2]赤峰市翁牛特旗农牧局,内蒙古翁牛特旗024500

出　　处：《北方农业学报》2021年第2期84-88,共5页Journal of Northern Agriculture

基　　金：国家食用豆产业技术体系项目(CARS-08-Z06);内蒙古农牧业创新基金项目(2016CXJJN06)。

摘　　要：【目的】明确绿豆晕疫病在内蒙古地区的发生情况及致病菌特性。【方法】2014—2018年实地调查了内蒙古地区28个旗(县)绿豆产区晕疫病发生情况,采集各绿豆产区病株进行病原菌分离,观察病原菌形态和菌落特征,测定其致病性、生理生化和碳源利用,并结合分子生物学技术对病原菌进行鉴定。【结果】28个旗(县)绿豆产区均有晕疫病发生,发病率为20%~100%。经过对病原菌表型观察、生理生化鉴定和分子生物学鉴定,分离菌株为丁香假单胞菌菜豆致病变种菌(Pseudomonas syringae pv.phaseolicola)。病原菌对碳源的利用程度为蔗糖>肌醇>葡萄糖>乳糖。【结论】内蒙古地区28个旗(县)绿豆产区均有晕疫病发生,致病菌为丁香假单胞菌菜豆致病变种菌(Pseudomonas syringae pv.phaseolicola)。【Objective】To clarify the occurrence of mung bean halo blight and the characteristics of pathogenic bacteria in Inner Mongolia.【Methods】The occurrence of halo blight in mung bean producing areas of 28 banners(counties)in Inner Mongolia from 2014 to 2018 was investigated.The pathogenic bacteria were isolated from the infected mung bean,and the morphology and colony characteristics of the pathogenic bacteria were observed.The pathogenicity,physiological and biochemical characteristics and carbon source utilization were determined,and the pathogenic bacteria were identified combined with molecular biological technology.【Results】The halo blight occurred in all the 28 banners(counties)and the incidence rate was 20%-100%.The strain was identified as Pseudomonas syringae pv.phaseolicola after phenotypic observation,physiological and biochemical identification and molecular biological identification.The utilization degree of carbon source was sucrose>inositol>glucose>lactose.【Conclusion】The halo blight occurred in all the 28 banners(counties)in Inner Mongolia,the pathogenic bacteria was Pseudomonas syringae pv.phaseolicola.

关 键 词：绿豆 晕疫病 病原菌 鉴定 

分 类 号：S435.22[农业科学—农业昆虫与害虫防治]