精浆甘油三酯含量对猪精液保存效果的影响  

Effect of the Content of Triglyceride in Seminal Plasma on the Preservation of Pig Semen

作　　者：甘麦邻 刘麟 郑婷 赵雪[1,2] 郭宗义 沈林園[1,2] 潘红梅[3] 张亮 王金勇[3] 朱砺[1,2] GAN Mailin;LIU Lin;ZHENG Ting;ZHAO Xue;GUO Zongyi;SHEN Linyuan;PAN Hongmei;ZHANG Liang;WANG Jinyong;ZHU Li(College of Animal Science and Technology,Sichuan Agricultural University,Sichuan Chengdu 611130,China;Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,Sichuan Agricultural University,Sichuan Chengdu 611130,China;Chongqing Academy of Animal Science,Chongqing 402460,China)

机构地区：[1]四川农业大学动物科技学院,四川成都611130 [2]四川农业大学畜禽遗传资源发掘与创新利用四川省重点实验室,四川成都611130 [3]重庆市畜牧科学院,重庆402460

出　　处：《中国畜牧杂志》2021年第6期181-186,共6页Chinese Journal of Animal Science

基　　金：国家重点研发计划项目(2018YFD0501004);国家现代农业产业技术体系四川生猪创新团队项目(SCSZTD-3-008);四川省科技支撑计划项目(2016NYZ0050);重庆市社会事业与民生保障科技创新专项项目(cstc2017-zdyf0247)。

摘　　要：为探究甘油三酯(TG)含量对猪精液冷冻保存效果的影响,本实验测定公猪精浆TG含量,并采用常温(17℃)和超低温冷冻(-196℃)2种方式对精液进行保存,测定精液保存时间和精子质量指标。结果显示:精液TG含量与冷冻保存精液精子活率负相关(P<0.05),与冷冻保存精子活力、快速前进运动精子和慢速前进运动精子存在负相关趋势(0.05<P<0.1);精浆高TG(0.81 mmol/L)组的果糖、过氧化氢、总蛋白含量高于低TG(0.26 mmol/L)组(P<0.05),冷冻保存后高TG组精子活率低于低TG组(P<0.05),精子活率和活力下降比率高于低TG组(P<0.05)。可见,精浆中较高浓度的TG含量可能对猪精液冷冻保存存在不利影响。In order to explore the effect of triglyceride(TG)content on the effect of pig semen cryopreservation,boar semen with different triglyceride content was stored at 17℃and cryopreservation(-196℃).TG level,storage time and sperm index were determined.The results showed that semen TG content was significantly negatively correlated with cryopreserved sperm viability,and there was a negative correlation trend(0.05<P<0.1)with cryopreserved sperm motility,fast-moving sperm and slow-moving sperm.The content of fructose,H2O2 and total protein in the seminal plasma with high TG content group(0.81±0.18 mmol/L)was significantly higher(P<0.05)than that in the low TG group(0.26±0.03 mmol/L).After cryopreservation,the sperm viability of the high TG content group was significantly(P<0.05)lower than that of the low TG group,and the sperm viability and motility decline rate were also significantly higher(P<0.05)than the low TG group.The higher TG content in seminal plasma may have an adverse effect on the cryopreservation of pig semen.

关 键 词：猪 精液 甘油三酯 常温保存 冷冻保存 

分 类 号：S828.3[农业科学—畜牧学]