补肾清肝疏脾法对2型糖尿病SD大鼠模型肠道菌群改善及MAPK信号通路的影响  

Effect of Bushen Qinggan Shupi on Intestinal Flora Improvement and MAPK Signaling Pathway in Type 2 Diabetes Model Rats

作　　者：范艳艳 陈晟 邢俊 陈劲宇 FAN Yanyan;CHEN Cheng;XING Jun;CHEN Jinyu(Department of Emergency Medicine,Nantong Hospital of Traditional Chinese Medicine,Nantong 226001,China;Department of Orthopaedics,Nantong first People's Hospital,Nantong 226001,China)

机构地区：[1]江苏省南通市中医院急诊内科,南通226001 [2]南通市第一人民医院骨科,南通226001

出　　处：《实验动物科学》2021年第2期17-21,共5页Laboratory Animal Science

基　　金：南通市卫生局青年科研基金(No.WKZL2018010)。

摘　　要：目的本研究拟评估补肾清肝疏脾法对2型糖尿病SD大鼠模型的治疗作用及其对MAPK信号通路的影响。方法选用2型糖尿病SD大鼠模型,分为模型对照组(对照组,n=8)和补肾清肝疏脾法组(实验组,n=8)。实验组大鼠采用蒸馏水制备的中药方剂,对照组和正常饲养组(空白组,n=8)使用无菌水,连续灌胃6个月,然后采集样品,进行肠道菌群、胰高血糖素样肽-1(GLP-1)、酪酪肽(PYY)含量、体质量、空腹血糖(FBG)、血脂及胰岛素分泌指数的分析。结果对照组大鼠肠道内双歧杆菌和乳酸杆菌显著降低、而粪肠球菌及大肠杆菌有明显的增加。使用补肾清肝疏脾法对大鼠进行治疗后,Western bolt结果显示,糖尿病模型大鼠肠道的GLP-1含量较治疗前有显著性差异。ELISA结果显示,空白组与对照组大鼠相比,PYY含量未见显著性变化;而实验组大鼠其含量增加显著。实验组大鼠的血糖、血脂、HOMA-β、体质量与对照组相比,在3个月、6个月均有显著性差异。结论补肾清肝疏脾法可控制糖尿病的发展,其作用机制可能是通过改善肠道菌群、GLP-1和PYY含量进行的。Objective To evaluate the therapeutic effect of Bushen Qinggan Shupi method on SD rats with type 2 diabetes.Method Diabetic rats were divided into diabetic model control group(control group,n=8),diabetic model Bushen Qinggan Shupi group(experimental group,n=8).Rats in the experimental group were infused with traditional Chinese medicine prescription prepared with distilled water,while rats in control group and normal feeding group(blank group,n=8)were infused with sterile water.Six months later,samples were collected for intestine.Result Bacterial flora,GLP-1,PYY body weight,fasting blood glucose(FBG),blood lipid,and insulin secretion index were analyzed.Compared with the blank group,Bifidobacteria and Lactobacilli in the intestines of rats in the experimental group decreased significantly,while Enterococcus faecalis and Escherichia coli increased significantly.The result of Western bolt showed that the content of GLP-1 in the intestine of diabetic ratswas significantly different from that of untreated rats.The result of ELISA showed that there was no significantchange in PYY content between the blank group and the diabetic model control group,but the PYY content of the blank group increased significantly after the treatment of invigorating the kidney,clearing the liver and dispersing the spleen.There were significant differences in blood glucose,blood lipid,HOMA-βand body weight of diabetic rats before and after intragastric administration of kidney tonifying liver dispersing spleen.Conclusion The method of strengthening kidney,clearing liver and dispersing spleen has a certain control effect on diabetes mellitus,the mechanism of which may be through the improvement of intestinal flora and the content of GLP-1,PYY content.

关 键 词：肠道菌群 2型糖尿病 清肝疏脾 MAPK 

分 类 号：R285.5[医药卫生—中药学]