氢气对实验性小鼠过敏性鼻炎减轻作用的研究  

Research on the Alleviating Effect of Hydrogen Gas on Allergic Rhinitis in Mice

作　　者：严伟明 龙盘 杨健松 危冬昱 张宇飞[1] 陈梅珠 张作明[1] 陈涛[1] YAN Weiming;LONG Pan;YANG Jiansong;WEI Dongyu;ZHANG Yufei;CHEN Meizhu;ZHANG Zuoming;CHEN Tao(Center of Clinical Aerospace Medicine,Air Force Medical University,Xi’an 710032,China;Department of Ophthalmology,The 900th Hospital of Joint Logistic Support Force,PLA,Clinical Medical College of Fujian Medical University,Dongfang Hopsital Affiliated to Xiamen University,Fuzhou 350025,China;Department of Ophthalmology,The General Hospital of Western Theatre Command,PLA,Chengdu 610083,China;The Health Center of the 94755 Troops,PLA,Zhangzhou 363900,China)

机构地区：[1]中国人民解放军空军军医大学航空航天临床医学中心,西安710032 [2]中国人民解放军联勤保障部队第九〇〇医院,福建医科大学福总临床医学院,厦门大学附属东方医院,福州350025 [3]中国人民解放军西部战区总医院,成都610083 [4]中国人民解放军94755部队卫生所,漳州363000

出　　处：《实验动物科学》2021年第2期35-41,共7页Laboratory Animal Science

基　　金：陕西省重点研发计划项目(No.2018SF-257);联勤保障部队第九〇〇医院院内课题杰出青年培育专项(No.2018Q02);福建省科技重点项目基金资助(No.2016Y0067)。

摘　　要：目的观察氢气能否减轻小鼠过敏性鼻炎。方法BALB/c雌鼠24只,随机分成正常组、模型组、氮氧组、氢氧组。除正常组外,其余三组小鼠,前14 d内,每2天一次,腹腔注射溶有50μg卵白蛋白(ovalbumin,OVA)+2 mg氢氧化铝[Al(OH)3]的生理盐水0.3 mL;第15~21天给予两鼻腔内滴10μL内含200μg OVA生理盐水进行过敏性鼻炎造模。在造模过程中,氮氧组小鼠每天给予吸入氮氧混合气体(66.7%N_(2)+33.3%O_(2))2 h;氢气组每天给予吸氢氧混合气体(66.7%H_(2)+33.3%O_(2))2 h。从第15~21天,观察各组小鼠鼻腔给药后10 min内搔鼻子及打喷嚏次数,于第21天统一处死各组小鼠,行鼻腔病理组织切片苏木素-伊红(HE)染色,进行鼻黏膜内嗜酸性粒细胞计数。结果造模后,模型组和氮氧组小鼠搔鼻子次数较正常组增多,差异有统计学意义(P<0.05),而氢氧组小鼠搔鼻子次数与正常组间差异无统计学意义。造模后,模型组小鼠打喷嚏次数和鼻腔黏膜嗜酸性粒细胞较正常组明显增多(P<0.05),而氢氧组小鼠打喷嚏次数和鼻腔黏膜嗜酸性粒细胞计数较模型组、氮氧组少,差异有统计学意义(P<0.05)。结论氢气对小鼠过敏性鼻炎有一定的减轻作用。Objective To observe whether hydrogen gas can ameliorate the allergic rhinitis(AR)in mice.Method Twenty-four female BALB/c mice(7-weeks-old)were randomly divided into the normal group,the model group,the nitrogen-oxygen group,and the hydrogen-oxygen group.Except the normal group,mice from the other 3 groups were intraperitoneally injected with 0.3 mL saline dissolved with 50μg OVA and 2 mg aluminum every 2 days lasting for 2 weeks.From day 15 to day 21,10μL saline with 200μg OVA was administered to both nostrils.During the whole process,a mixed gas of 66.7%N_(2) and 33.3%O_(2)was given for 2 hours every day to the mice of the nitrogen-oxygen group,while those from the hydrogen-oxygen group inhaled a mixed gas of 66.7%H_(2) and 33.3%O_(2)at the same time.The number of nose scratching and sneezing of each mice was counted within 10 minutes after nasal administration from day 15 to day 21.On the 21^(st) day,mice of each group were sacrificed,and the histological sections of the nasal cavity with hematoxylin-eosin staining(HE)staining was performed with the nasal mucosal eosinophils counted.Result After modeling of AR,the number of nose-scratching in the model group and the nitrogen-oxygen group was increased compared with that the normal group with the difference being statistically significant(P<0.05).Meanwhile,the number of nose-scratching in the hydrogen-oxygen groups was not statistically significant from that of the normal group(P>0.05).The numbers of sneezing and nasal mucosal eosinophils in the model group were significantly increased compared with those of the normal group(P<0.05).Meanwhile,the numbers of sneezing and nasal mucosal eosinophils in the hydrogen-oxygen groups were significantly lower than those in both the model group and the nitrogen-oxygen groups(P<0.05).Conclusion Hydrogen gas could alleviate the allergic rhinitis in mice.

关 键 词：氢气 过敏性鼻炎 嗜酸性粒细胞 

分 类 号：R76[医药卫生—耳鼻咽喉科]