DRD1基因敲除影响雄鼠生育的分子机理研究  

Study on Molecular Mechanism of Male Mice Reproduction in DRD1 Knockout

作　　者：龚晓娟 马盼 孙敏 杨婧思 李少卿 王兴林 张平[1] 阎春霞[1] 张宝[1] GONG Xiaojuan;MA Pan;SUN Min;YANG Jingsi;LI Shaoqing;WANG Xinglin;ZHANG Ping;YAN Chunxia;ZHANG Bao(Key Lab of Ministry of Health Department of Forensic Medicine,Xi’an 710061,China;Key Lab of Environment and Diseases Relevant Genes of Ministry of Education,Xi’an Jiaotong University,Xi’an 710061,China)

机构地区：[1]西安交通大学卫生部法医学重点实验室,西安710061 [2]西安交通大学环境与疾病相关基因教育部重点实验室,西安710061

出　　处：《实验动物科学》2021年第2期53-56,60,共5页Laboratory Animal Science

基　　金：陕西省自然科学基金(No.2017JQ8010);中央高校基本科研业务费专项资金(No.xjj2017135)。

摘　　要：目的研究DRD1基因敲除引起雄鼠生殖功能下降的机制。方法本研究以DRD1基因敲除鼠(KO)和野生鼠(WT)为研究对象,对8周龄KO与WT雄鼠进行基因型鉴定,测体质量并计算睾丸系数,一侧睾丸HE染色观察形态,另一侧睾丸TRIzol法提取RNA,通过qRT-PCR反应,采用相对定量法,分析与生殖密切相关基因的表达差异倍数。结果KO与WT鼠体质量相比较具有显著差异(P<0.05)。睾丸组织病理学切片观察发现,相对于WT鼠,KO鼠生精小管排列疏松紊乱,生殖细胞层数少,提示精子形态及功能存在异常。通过qRT-PCR进行基因表达差异分析,KO小鼠相对WT小鼠CFTR表达上调2.32倍,Spata19上调4.58倍,SMCY下调5.25倍,DAZ下调3.14倍。结论以上研究发现,敲除DRD1基因可通过影响睾丸组织结构及相关基因表达,进而影响生殖。Objective To study the mechanism of declining reproduction between DRD1 gene knockout mice(KO)and wild mice(WT).Method Eight weeks male mice,including KO and WT mice,were weighted and the testis coefficient was calculating.The testis was observed by HE staining and reproduction-related genes were analyzed by qPCR.Result The result showed that KO and WT mice had significant difference in weight(P<0.05).The HE staining showed that the seminiferous tubule of KO mice was derangement and germ cell had less cellular layer,which suggesting morphology and function of sperms may be abnormal in KO mice.For geneticexpression,CFTR was up-regulated 2.32 fold,Spata19 was up-regulated 4.58 fold,SMCY was down-regulated 5.25 fold and DAZ was down-regulated 3.14 fold between KO and WT mice,revealing that declining reproduction in KO male mice may related with the expression of reproduction-related genes.Conclusion In conclusion,DRD1 gene could influence reproduction by genetic expression.

关 键 词：DRD1基因 基因敲除 生殖 分子机理 

分 类 号：Q75[生物学—分子生物学]