平痫冲剂及其拆方的抗痫作用以及对幼龄癫痫模型大鼠脑皮质基质金属蛋白酶-9、水通道蛋白-4表达的影响  

Antiepileptic effects of Pingxian Chongji(平痫冲剂)along with decomposed prescriptions thereofand effects on expression of MMP-9 and AQP-4 in cerebralcortex of young epileptic model rats

作　　者：高旅 尚菁 史正刚 吴丽萍 GAO Lyu;SHANG Jing;SHI Zhenggang;WU Liping(Key Laboratory for Eneephalopathy of Traditional Chinese Medicine in Shanxi Province,The Fourth ClinicalCollege of Shanxi University of Chinese Medicine,Taiyuan,Shanxi,030619,China;Department of Pediatrics,Shanxi Hospital of Integrated,Traditionual Chinese and Western Medicine,Taiyuan,Shanmxi,030001,China;Clinical College of TCM,Gansu University of Chinese Medicine,Lanzhou,Gansu,730101,China;Depariment of Pexliatrics,Affiliated Hospital of Gansu Universily of Chinese Medicine,Lanzhou,Gansu,730020,China)

机构地区：[1]山西中医药大学第四临床学院,中医脑病学山西省重点实验室,山西太原030619 [2]山西省中西医结合医院儿科,山西太原030001 [3]甘肃中医药大学中医临床学院,甘肃兰州730101 [4]甘肃中医药大学附属医院儿科,甘肃兰州730020

出　　处：《中医儿科杂志》2021年第3期8-15,共8页Journal of Pediatrics of Traditional Chinese Medicine

基　　金：国家自然科学基金面上基金项目(81460739)。

摘　　要：目的观察平痫冲剂及其拆方对幼龄癫痫模型大鼠的抗痫作用与脑皮质区超微结构及相关蛋白基质金属蛋白酶-9(MMP-9)、水通道蛋白-4(AQP-4)表达的影响。方法SPF级28日龄Wistar大鼠共450只,随机选取60只作为空白组,造模组390只,采用戊四唑腹腔注射制备癫痫模型。模型制备成功的360只大鼠随机分成模型组、西药组、化痰组、祛瘀组、息风组及平痫冲剂组,每组60只,分别予以药物灌胃21 d;各组大鼠随机选取10只,分别于干预24 h、48 h、72 h、7 d、14 d、21 d后,利用戊四唑腹腔注射诱导,观察1 h内各组大鼠惊厥发作情况,并取大鼠脑组织,进行皮质区超微结构观察及MMP-9、AQP-4表达的变化。结果与模型组比较,西药组在用药干预24 h、48 h MMP-9阳性表达减弱(P<0.05或P<0.01);化痰组在用药干预14 d MMP-9阳性表达减弱(P<0.05);息风组在用药干预7 d MMP-9阳性表达减弱(P<0.05);平痫冲剂组在用药干预24 h MMP-9阳性表达减弱(P<0.05),48 h作用最显著(P<0.01)。与同组用药干预24 h后比较,西药组在用药干预72 h和7 d MMP-9阳性表达减弱(P<0.05或P<0.01);化痰组在用药干预14 d MMP-9阳性表达减弱(P<0.05),至21 d MMP-9蛋白阳性表达显著减弱(P<0.01);平痫冲剂组在用药干预72 h MMP-9阳性表达明显减弱(P<0.01)。与各拆方组比较,平痫冲剂组在用药48 h后各时间段MMP-9表达均明显减弱(P<0.01)。与模型组比较,西药组在用药干预24 h、48 h AQP-4阳性表达减弱(P<0.05或P<0.01);化痰组在用药干预14 d AQP-4阳性表达减弱(P<0.05);息风组在用药干预72 hAQP-4阳性表达减弱(P<0.05);平痫冲剂组在用药干预48 h AQP-4阳性表达减弱(P<0.05),72 h作用最为显著(P<0.01)。与同组用药干预24 h比较,西药组在用药干预48 h、72 h AQP-4阳性表达减弱(P<0.05或P<0.01);平痫冲剂组在用药干预72 h AQP-4阳性表达明显减弱(P<0.01)。与各拆方组比较,平痫冲剂组在用药干预7 d AQP-4阳性表达减�Objective To observe the antiepileptic effects of Pingxian Chongji(平痫冲剂)along with decomposed prescriptions thereof and effects on expression on young epileptic model rats and effects on cerebral cortical area ultrastructure and expression of related proteinsmatrix metallopeptidase-9(MMP-9)and aquaporin-4(AQP-4).Methods Among 450 SPF Wistar rats aged 28 days,60 were randomly chosen as blank group,and 390 rats as establishing model group.The epilepsy model was prepared by intraperitoneal injection of pentetrazole.After the model was successfully established,the rats were randomly divided into model group,Western medicine group,phlegm resolving group,blood stasis removing group,stopping wind group and Pingxian Chongji group,with 60 rats in each group.They were given drugs by gavage for 21 days respectively.Ten rats were randomly chosen from each group,and were induced by intraperitoneal injection of pentetrazol after 24 hours,48 hours,72 hours,7 days,14 days and 21 days,respectively,the seizure attacks of rats in each group were observed within 1 hour,and the brain tissues of rats were taken to observe cortex area ultrastructureand expression of MMP-9 and AQP-4.Results Compared with the model group,the positive expression of MMP-9 in Western medicine group decreased at 24 hours and 48 hours after medication intervention(P<0.05 or P<0.01);the positive expression of MMP-9 in phlegm resolving group decreased on the 14 th day after medication intervention(P<0.05);The positive expression of MMP-9 in stopping wind group decreased on the 7 th day after medication intervention(P<0.05);The positive expression of MMP-9 in Pingxian Chongji group decreased at 24 hours after medication intervention(P<0.05),and the effect was the most significant at 48 hours(P<0.01).Compared with the same group at 24 hours after medication intervention,the positive expression of MMP-9 in Western medicine group decreased at 72 hours and on the 7 th day after medication intervention(P<0.05 or P<0.01);the positive expression of MMP-9 in phl

关 键 词：癫痫 平痫冲剂 拆方研究 基质金属蛋白酶-9 水通道蛋白-4 实验研究 

分 类 号：R285.5[医药卫生—中药学]