黄连根腐病腐霉属病原菌鉴定  

Identification of the Pythium Pathogen Responsible for Root Rot of Coptis chinensis

作　　者：伍晓丽[1,2,3,4] 陈大霞 刘飞[2,5] 王钰 李隆云[1,2,3,4] WU Xiao-li;CHEN Da-xia;LIU Fei;WANG Yu;LI Long-yun(Institute of Material Medical Planting,Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China;Chongqing Sub-center of National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Science,Chongqing 400065,China;Chongqing Engineering Research Center for Fine Variety Breedling Techniques of Chinese Materia Medica,Chongqing 400065,China;Chongqing Key Laboratory of Traditional Chinese Medicine Rcsource,Chongqing 400065,China;Health Center of Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China)

机构地区：[1]重庆市中药研究院种植研究所,重庆400065 [2]中国中医科学院中药资源中心重庆分中心,重庆400065 [3]重庆市中药良种选育与评价工程技术研究中心,重庆400065 [4]中药资源学重庆市重点实验室,重庆400065 [5]重庆市中药研究院大健康中心,重庆400065

出　　处：《西南大学学报（自然科学版）》2021年第6期37-43,共7页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市基本科研业务费项目(cstc2019jxjl-jbky10004);重庆市卫计委重点项目(ZY201801006);重庆市自然科学基金项目(cstc2019jcyj-msxmX0677);农业农村部国家中药材产业技术体系项目(CARS-21);科技部国家重点研发计划项目(2017YFC1702601).

摘　　要：黄连是我国的重要中药材,根腐病是黄连的主要病害之一,近几年大面积爆发,给生产造成了严重损失.为了明确其病原菌,采用组织分离法分离出病根中的腐霉属真菌,通过离体回接初步筛选出疑似病原菌,再通过活体回接明确病原菌,利用分子鉴定和形态鉴定将病原菌鉴定到种,并筛选出病原菌菌株的最适合培养基.结果共计分离到腐霉属真菌8株,其中Pythium.macrosporum在室温(日均温4.7~11.2℃)和25℃离体回接和活体回接时均显示致病性.根据以上结果推断,P.macrosporum是病原菌之一,且具有在高温和低温都能致病的能力.纯脱皮小麦粒是P.macrosporum的最佳培养基.Coptis chinensis is an important traditional Chinese medicine herb,and root rot is one of its main diseases,which has repeatedly broken out in some producing areas in recent years,causing severe damage to the crop.To identify the pathogen(s)of this disease,according to Kochs postulate,Pythium spp.strains were isolated from the rot root.Then,probable pathogens were preliminarily screened with the method of in vitro back inoculation,and identified with molecular and morphological method.In the result,8 Pythium strains were obtained,of which P.macrosporum displayed pathogenicity when in vitro inoculated on the root and the plant at both room temperature(daily mean temperature 4.7~11.2℃)and 25℃.So P.macrosporum was speculated to be one of the pathogens and could infect C.chinensis at both low and high temperature.Pure decorticated wheat was the best medium for P.macrosporum.

关 键 词：黄连根腐病 腐霉属 病原菌 

分 类 号：S888.716[农业科学—特种经济动物饲养]