基于高密度SNP标记对玉米籽粒相关性状的QTL定位  

QTL Mapping About Kernel-Related Traits in Maize Based on High Density SNP Markers

作　　者：王安贵[1] 赵强 吴迅 郭向阳[1] 刘鹏飞 祝云芳[1] 陈泽辉[1] Wang Angui;Zhao Qiang;Wu Xun;Guo Xiangyang;Liu Pengfei;Zhu Yunfang;Chen Zehui(Institute of Upland Crops,Guizhou Academy of Agricultural Sciences,Guiyang,550006)

机构地区：[1]贵州省农业科学院旱粮研究所,贵阳550006

出　　处：《分子植物育种》2021年第10期3323-3328,共6页Molecular Plant Breeding

基　　金：贵州省重点基金项目(黔科合基础[2020]1Z019;黔科合基础[2017]1413);国家“七大作物育种”专项项目(2016YFD0101206-4,2018YFD0100104);国家自然科学基金项目(31760387);贵州省高层次人才项目(黔科合平台人才[2018]5629);中央引领地方科技发展专项项目(黔科中引地[2018]4003)共同资助。

摘　　要：高产是玉米育种的主要目标之一,而玉米籽粒是产量构成最重要的因子。本研究以‘T32’与‘齐319’为亲本构建118份F2:3家系的双亲分离群体为材料,通过对不同家系的3个籽粒性状(粒长,粒宽和粒重)进行评价,结合高密度SNP标记的基因型鉴定结果,利用IciMapping 4.2软件中的复合区间作图法对粒长、粒宽和粒重进行了QTL定位。共检测到11个QTL,其中控制粒重、粒长和粒宽的QTL分别为2、2和7个,单个QTL可解释4.03%~11.34%的表型变异。研究结果将为玉米籽粒性状的遗传改良提供更多的遗传位点,也为后续的精细定位以及候选基因功能研究提供科学支持。High yield is one of the main goals of maize breeding,and maize kernel is the most important factor of yield composition.’T32’and’Qi319’were used as parents to construct 118 F2:3 families.Kernel related traits(kernel length,kernel width and kernel weight)of different families were evaluated,and genotype of each plants were identified by using high-density SNP markers.QTL mapping was conducted for kernel length,kernel width and kernel weight by using composite interval mapping method in IciMapping 4.2 software.A total of 11 QTLs were detected,wherein 2,2 and 7 QTLs were detected to be related with kernel weight,kernel length and kernel width.A single QTL could explain phenotypic variation of 4.03%~11.34%.These results would provided much more genetic locus for the genetic improvement of ear traits in corn,and some reliable candidate regions for the exploration of candidate genes’function.

关 键 词：玉米 籽粒性状 SNP标记 QTL 

分 类 号：S513[农业科学—作物学]