食用羽衣甘蓝‘沪羽甘1号’品种选育及其亲本材料DNA指纹图谱构建  

Breeding of Edible Kale’Huyugan No.1’and Construction of DNA Fingerprint of Parents

作　　者：邰翔[1] 陈锦秀[1] 朱晓炜 宋云鹏[1] 薄天岳[1] Tai Xiang;Chen Jinxiu;Zhu Xiaowei;Song Yunpeng;Bo Tianyue(Horticultural Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai,201106)

机构地区：[1]上海市农业科学院园艺研究所,上海201106

出　　处：《分子植物育种》2021年第10期3344-3349,共6页Molecular Plant Breeding

基　　金：“十三五”国家科技重点研发计划项目(2017YFD0101804);上海市科技兴农攻关项目(沪农科攻字[2015]第6-1-7号);国家现代农业产业技术体系建设专项资金项目(CARS-25)共同资助。

摘　　要：食用羽衣甘蓝新品种‘沪羽甘1号’是利用羽衣甘蓝胞质雄性不育系与高代自交系通过常规育种技术选育而成的F1代杂交种,2019年通过上海市农作物品种审定委员会认定。为进一步推广和保护该品种,以‘沃特斯’品种为对照,比较研究了‘沪羽甘1号’的特征特性,并利用ISSR分子标记技术构建了‘沪羽甘1号’亲本的DNA指纹图谱。与对照‘沃特斯’相比,‘沪羽甘1号’具有较高的营养价值,且具有早熟、抗黑腐病的特点。‘沪羽甘1号’蛋白质、VC、纤维素、水分和可溶性固形物的含量分别为20.7%、95.1(100 mg/kg)、10.8%、76.1%和8.8%。从80个ISSR引物中筛选出8条核心引物在‘沪羽甘1号’父本‘HL2250-8’和母本‘CMS-015-319’中分别扩增出10和13条多态性条带,分别选择其中的5和7条谱带构建‘HL2250-8’和‘CMS-015-319’的DNA指纹图谱。该研究结果为‘沪羽甘1号’推广和品种保护提供了科学依据。A ne w edible kale cultivar’Huyugan No.1’is an F1 hybrid selected through conventional breeding techniques using a kale CMS line and high generation inbred line.Approved by the Shanghai Crop Variety Review Committee in 2019.In order to promote this variety,the characteristics of’Huyugan No.1’were compared to the’Waters’variety,and the DNA fingerprints of the parents of’Huyugan No.1’were constructed using ISSR molecular marker.Compared with the control,’Huyugan No.1’has higher nutritional value,early maturity and resistance to black rot.The results showed that the content of protein,VC,cellulose,water and soluble solids in’Huyugan No.1’were 20.7%,95.1(100 mg/kg),10.8%,76.1%and 8.8%,respectively.Eight core primers were selected from 80 ISSR primers,and 10 and 13 polymorphic bands were amplified in the male parent’HL2250-8’and the female parent’CMS-015-319’of’Huyugan No.1’,5 and 7 bands were selected respectively to construct the DNA fingerprints of’HL2250-8’and’CMS-015-319’.The results provide a scientific basis for the promotion and variety protection of’Huyugan No.1’.

关 键 词：食用羽衣甘蓝 选育 ‘沪羽甘1号’ 

分 类 号：S635[农业科学—蔬菜学]