醉蝶花种子化学成分提取及GC-MS分析  

On Extraction and GC-MS Analysis of Chemical Constituents from Seeds of Pieris

作　　者：覃彪 刘杨 王勇 QIN Biao;LIU Yang;WANG Yong(College of Chemistry and Chemical Engineering, Zunyi Normal University, Zunyi Guizhou 563006, China)

机构地区：[1]遵义师范学院化学化工学院,贵州遵义563006

出　　处：《西南师范大学学报（自然科学版）》2021年第5期32-37,共6页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：遵义市科技局、遵义师范学院联合科技研发资金项目(遵市科合计[2018]05号);贵州省科技厅基础研究计划项目(黔科合基础[2017]1199号).

摘　　要：以醉蝶花种子为研究材料,用超声波提取技术对醉蝶花种子成分进行提取,通过单因素试验和正交试验探究了不同的提取时间、提取温度和料液比等因素对提取率的影响,用气相色谱质谱分析(gas chromatography-mass spectrometry,GC-MS)技术对提取物进行分析检测.结果表明,在固定超声功率为300 W,提取时间为24 min,提取温度为38℃,料液质量体积比(g/mL)为1∶12的提取条件下,醉蝶花种子成分提取率可达17.84%.醉蝶花种子用丙酮作浸提剂的超声波提取法提取的主要物质是醇类化合物,含量高达66.48%.超声波提取法操作简便,效率高,成本相对较低,是一种对醉蝶花种子有效成分提取的可行方法.The components of the spiderflower seeds were extracted by ultrasonic technology,with single factor and orthogonal experiments,the influence of different extraction time,extraction temperature and material to liquid ratio were studied,and the extraction analyzed by GC-MS.The results show that under the conditions of ultrasonic power 300 W,extraction time 24 min,extraction temperature 38℃and ratio of material to liquid 1:12,the extraction rate of the spiderflower seeds was 17.84%.The main substance extracted by ultrasonic method with acetone was the alcohol as content of 66.48%.The ultrasonic extraction method is simple,high efficiency and low cost.It is a feasible method to extract the effective components from the seeds of spiderflower seeds.

关 键 词：超声波提取 醉蝶花 种子 气相色谱质谱分析(GC-MS) 

分 类 号：O657.7[理学—分析化学]