普鲁卡因对牛蛙离体坐骨神经干电生理学特性的影响  

Effects of Procaine on Electrophysiological Properties of Bullfrog’s Sciatic Nerve Trunk

作　　者：陈德多 王敏鑫 李娇 姜博奥 师小贻 徐长婷 顾丹 金会艳 边慧 Chen Deduo;Wang Minxin;Li Jiao;Jiang Boao;Shi Xiaoyi;Xu Changting;Gu Dan;Jin Huiyan;Bian Hui(Schoo1 of Basic Medical Science,Kunming Medical University,Kunming 650500,China)

机构地区：[1]昆明医科大学基础医学院,云南昆明650500

出　　处：《广东化工》2021年第9期121-122,129,共3页Guangdong Chemical Industry

基　　金：国家自然科学基金(编号:81760212);云南省科技厅-昆明医科大学应用基础研究联合资金项目(编号:202001AY070001-016);云南省卫生健康委员会医学后备人才培养计划(编号:H-2018054);云南省神经系统疾病诊疗中心应用基础研究专项基金子项目(编号:ZX 2019-03-05)。

摘　　要：目的:观察普鲁卡因对牛蛙离体坐骨神经干电生理学特性的影响。方法:将制备好的牛蛙离体坐骨神经干置于任氏液浸泡20 min,随机分为2组:即任氏液对照组和普鲁卡因实验组,实验组神经干浸泡普鲁卡因1 min。分别测定两组坐骨神经干传导速度、神经干复合动作电位幅度、阈强度和最大刺激强度、绝对不应期和相对不应期等电生理学指标。结果:经普鲁卡因处理后,坐骨神经干传导速度明显减慢,神经干复合动作电位幅度明显减小;神经干阈强度和最大刺激强度明显增大;神经干绝对不应期和相对不应期明显延长。结论:普鲁卡因可减慢牛蛙离体坐骨神经干传导速度,降低神经干兴奋性。Objective: To observe the effect of procaine on the electrophysiological properties of bullfrog’s sciatic nerve trunk in vitro. Methods: The prepared sciatic nerve trunk was immersed in Ringer’s solution for 20 minutes, and then randomly divided into two groups: Ringer’s solution control group and procaine experimental group, the nerve trunk of the experimental group was immersed in procaine for 1 min. The conduction velocity of sciatic nerve trunk, amplitude of nerve trunk compound action potential, threshold intensity and maximum stimulation intensity, absolute refractory period and relative refractory period were measured. Results: The conduction velocity of the sciatic nerve trunk was significantly slowed down, the amplitude of compound action potential of nerve trunk was significantly reduced, the threshold intensity and maximum stimulation intensity of nerve trunk were significantly increased, and the absolute refractory period and relative refractory period of nerve trunk were significantly prolonged after procaine treatment. Conclusion: Procaine can slow down the conduction velocity of bullfrog’s sciatic nerve trunk and reduce the excitability of the nerve trunk.

关 键 词：普鲁卡因 牛蛙 坐骨神经干 动作电位 

分 类 号：Q42[生物学—神经生物学] Q424[生物学—生理学]