分光光度法测定硫酸卡那霉素  

Determination of Kanamycin by Spectrophotometry

作　　者：郭庆 蒙秋红 吴子华 Guo Qing;Meng Qiuhong;Wu Zihua(Guangxi Teachers Education University,School of Chemistry and Materials Science,Nanning 541004;School of Chemistry and Chemical Engineering,South China University of Technology,Guangzhou 510641,China)

机构地区：[1]南宁师范大学广西天然高分子化学与物理重点实验室,广西南宁541004 [2]华南理工大学化学与化工学院,广东广州510641

出　　处：《广东化工》2021年第9期230-232,共3页Guangdong Chemical Industry

基　　金：国家自然科学基金51762008;广西自然科学基金2020GXNSFBA159041。

摘　　要：本文采用分光光度法对硫酸卡那霉素与二价铜离子结合形成的铜(П)-卡那霉素络合物的显色反应进行了定量分析。结果表明,CuSO_(4)浓度为0.050 g/L时,硫酸卡那霉素的浓度在0.010~0.360 g/L范围内与吸光度呈良好的线性关系,线性相关系数为0.9994,检出限为0.523 mg/L,加标回收率为98.7%~99.6%。将该方法用于介孔二氧化硅包覆金纳米棒结构吸附卡那霉素含量的测定,显示出良好的适用性。In this paper, the color reaction of copper(П)-kanamycin complex formed by the combination of kanamycin sulfate and Cu^(2+) was quantitatively analyzed by spectrophotometry. The experimental results show that there was a good linear relationship between kanamycin sulfate concentration and absorbance in the range of 0.050~0.400 g/L when CuSO_(4) concentration was 0.050 g/L, the linear correlation coefficient was 0.9994, the detection limit was 0.523 mg/L, and the recovery was 98.7 %~99.6 %. The method was applied to the determination of kanamycin content in gold nanorods coated with mesoporous silica, and showed good applicability.

关 键 词：分光光度法 硫酸卡那霉素 硫酸铜 线性关系 介孔二氧化硅 

分 类 号：TQ[化学工程]