Tongdu Tiaoshen Acupuncture Method for Promoting Hippocampal Nerve Repair and Regeneration and Improving Learning and Memory Dysfunction in Stroke Model Rats  

作　　者：Jin XIE Jingping MU Zhongmin HUANG Fangfang YANG Wanfen WANG Jimin LIU Jing TAO Lidian CHEN 

机构地区：[1]Shiyan Taihe Hospital (Affiliated Hospital of Hubei University of Medicine), Shiyan 442000, China [2]Fujian University of Traditional Chinese Medicine, Fuzhou 350000, China

出　　处：《Medicinal Plant》2021年第2期95-98,101,共5页药用植物：英文版

基　　金：Research Project of Health Commission of Hubei Province(WJ2017F072):Study on Brain Function Imaging Mechanism of Scalp Acupuncture Treatment of Post-Stroke Depression.

摘　　要：[Objectives]To evaluate the effects of Tongdu Tiaoshen Acupuncture method on improving learning and memory function and hippocampal nerve regeneration and repair after stroke.[Methods]Animal models of cognitive impairment in the recovery phase of cerebral infarction in rats were prepared,and rats were randomly divided into control group,model group,donepezil group,and acupuncture group.Morris water maze experiment was carried out to evaluate learning and memory ability.Nissl staining,BrdU and DCX immunofluorescence staining were used to observe the number and morphological structure of neuronal pyramidal and granular cells in the dentate gyrus(DG)area of the hippocampus,as well as the proliferation and differentiation of neural progenitor cells.TUNEL method was used to detect cell apoptosis.[Results]Before intervention,the scores and ELP of the model group,donepezil group and acupuncture group were significantly higher than those of the blank group(P<0.05);after the intervention,the neurological scores and ELP of the blank group and the model group were not significantly different from those before the intervention(P<0.05);the donepezil group and acupuncture group were significantly reduced,there was no difference between the donepezil group and acupuncture group(P>0.05),but it was still significantly higher than the blank group(P<0.05).The results of BrdU and DCX immunofluorescence staining showed that there were only a few BrdU positive cells and DCX protein in the hippocampal DG area of mice in the blank group,and the positive cells in the model group were significantly increased(P<0.05);donepezil group and acupuncture group showed significant increase compared with the model group(P<0.05);compared with the blank group,the number of neurons in the hippocampal DG area of each group was significantly reduced(P<0.01),and the neuronal apoptosis index was significantly increased(P<0.01);compared with the blank group,the number of neurons in the hippocampal DG area of each group was significantly reduced(P<0.01),and

关 键 词：Tongdu Tiaoshen ACUPUNCTURE STROKE Cognitive impairment Learning and memory function Hippocampal nerve 

分 类 号：R24[医药卫生—中医临床基础]