不同浓度TDZ和2,4-D组合对草莓花药组织培养的影响  

Effects of Different Concentrations TDZ and 2,4-D Combination on Anther Tissue Culture of Strawberry

作　　者：杜梦卿 连朋 王丽娟[1] DU Mengqing;LIAN Peng;WANG Lijuan(Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]天津农学院,天津300384

出　　处：《东北农业科学》2021年第2期73-75,131,共4页Journal of Northeast Agricultural Sciences

基　　金：天津市种业重大项目(17ZXZYNC00080);天津市科技成果转化与推广项目(201803010);天津市优秀科技特派员项目(19JCTPJC58200)。

摘　　要：为探究草莓花药离体再生体系最适宜培养条件,以草莓品种"枥乙女"花药为试材,研究细胞分裂素TDZ和生长素2,4-D对草莓花药愈伤组织诱导、分化和增殖的影响,筛选不同阶段最适宜草莓花药组织培养激素配比。结果表明,TDZ和2,4-D激素组合可成功培养出草莓花药再生植株,最适宜诱导培养基为MS+2,4-D 0.2 mg/L+TDZ 1.5 mg/L,最适宜分化培养基为MS+2,4-D 0.05 mg/L+TDZ 2.0 mg/L,最适宜增殖阶段培养基为MS+TDZ 0.5 mg/L+2,4-D 0.1 mg/L。In order to explore the most suitable culture conditions of strawberry anther in vitro regeneration system,the effects of cytokine TDZ and auxin 2,4-D on callus induction, differentiation and proliferation of strawberry anther were studied, and the most suitable hormone ratio in different stages of strawberry anther culture were selected with the strawberry cultivar "Tochiotome " anther as test material. The results showed that the combination of TDZ and 2,4-D could successfully culture Strawberry Anther regeneration plants. The most suitable induction medium was MS+2,4-D 0.2 mg/L+TDZ 1.5 mg/L. The most suitable differentiation medium was MS+2,4-D 0.05 mg/L+TDZ2.0 mg/L. The most suitable proliferation medium was MS+TDZ 0.5 mg/L+2,4-D 0.1 mg/L.

关 键 词：草莓 花药 培养基 激素 浓度 

分 类 号：S668.4[农业科学—果树学]