连翘、黄芩、大青叶三味中药药理作用及主要活性成分含量检测方法研究  

Study on the Pharmacologic Effects and Determinnation of Main Active Components in Forsythia, Radix Scutellariae and Folium Isatidis

作　　者：孔维洁 翟莹 袁发浒[1] 张涛[1] 曹晓琴[1] 陈中强 Kong Weijie;Zhai Ying;Yuan Fahu;Zhang Tao;Cao Xiaoqin;Chen Zhongqiang(School of Medcine,Jianghan University,Wuhan 430056,China)

机构地区：[1]江汉大学医学院,湖北武汉430056

出　　处：《广东化工》2021年第10期199-201,208,共4页Guangdong Chemical Industry

摘　　要：目的:对连翘、黄芩、大青叶三味中药主要化学物质及药理活性进行综述,并对三味药材的主要活性成分含量检测方法进行研究;方法:采用HPLC法测定,使用Kromasil C18(250 mm×4.6 mm,5μm)色谱柱;流动相为0.5%三乙胺(磷酸调PH=3)-乙腈溶液,梯度洗脱;流速为1.0 mL/min;柱温35℃;检测波长277 nm。结果:该方法精密度、稳定性、重复性试验的RSD均小于2.2%;连翘苷、黄芩苷、靛玉红在0.03~0.962 mg/mL(r=0.9995)、0.047~1.502 mg/mL(r=0.9991)、0.050~1.600 mg/mL(r=0.9992)质量浓度范围内呈现良好的线性关系,平均加样回收率分别为100.23%、101.28%、100.72%,RSD分别为1.72%、1.39%、1.98%(n=6)。结论:该方法高效、简便,专属性强,稳定性好,重复性好,可同时对含有连翘苷、黄芩苷、靛玉红三种成份的不同剂型的含量进行检测。Objective: To review the main chemical substances and pharmacological activities of Forsythia, Radix Scutellariae and folium isatidis, and to study the determination method of the main active components in the three herbs. Methods: HPLC method was used to determine. The chromatographic column was Kromasil C18(250 mm×4.6 mm, 5 μm), the mobile phase was 0.5% triethylamine(pH adjusted by phosphoric acid =3)-acetonitrile solution( gradient elution), the flow rate was 1.0 m L/min., the column temperature was 35 ℃ and the detection wavelength was 277 nm. Result:The RSDs of precision, stability and reproducibility tests were less than 2.2 %. The liner ranges of forsythin, baicalin and indirubin were 0.03~0.962 mg/mL(r=0.9995), 0.047~1.502 mg/mL(r=0.9991) and 0.050~1.600 mg/m L(r=0.9992), respectively. The average recoveries of forsythin, baicalin and indirubin were 100.23 %, 101.28 %, 100.7 %, with RSDs of 1.72 %, 1.39 %,1.98 %(n=6), respectively. Conclusion: The method is efficient, simple, specific, stable and reproducible, which can be used for the simultaneous determination of forsythia, baicalin and indirubin in different dosage forms.

关 键 词：连翘 黄芩 大青叶 高效液相色谱法 含量检测 

分 类 号：TQ[化学工程]