白细胞介素33通过促进上皮细胞黏附分子表达参与干燥综合征发病  

Interleukin-33 Involves in Pathogenesis of Sjogren’s Syndrome through Promoting Epithelial Cell Adhesion Molecule Expression

作　　者：周雅馨 李红霞 蔡鑫 符向辉 张葵 朱平 吴振彪 ZHOU Ya-xin;LI Hong-xia;CAI Xin;FU Xiang-hui;ZHANG Kui;ZHU Ping;WUZhen-biao(Department of Clinical Immunology,First Affiliated Hospital,Air Force Medical University,General PLA Research Institute of Rheumatology,Xi'an,Shaanxi,710032,China)

机构地区：[1]中国人民解放军空军军医大学第一附属医院临床免疫科全军风湿免疫专科研究所,陕西西安710032

出　　处：《现代生物医学进展》2021年第10期1824-1828,共5页Progress in Modern Biomedicine

基　　金：陕西省科技统筹创新工程计划项目(2016KTCL03-03)。

摘　　要：目的:探讨白细胞介素(Interleukin-33,IL-33)可能通过调控上皮细胞黏附分子(Epithelial cell adhesion molecule,EpCAM)表达参与干燥综合征(Sjogren's syndrome,SS)发病的作用机制.方法:收集因SS诊断需要行唇腺活检术的患者血清和唇腺组织标本及相关临床资料,根据2016年ACR-EULAR SS分类诊断标准将患者分为SS组和非SS组,选取性别、年龄匹配的21例SS患者和21例非SS患者,利用多重检测流式试剂盒(人炎症因子组合1)检测血清中IL-33水平并用t检验比较SS患者组与非SS患者组、抗SSA抗体阳性与阴性组以及唇腺病理阳性与阴性组之间IL-33水平的差异.对唇腺组织石蜡切片进行IL-33免疫组织化学染色,用流式细胞术检测新鲜唇腺组织中上皮细胞EpCAM表达水平并与血清IL-33水平进行相关性分析.结果:SS患者组IL-33水平为(1736±590.1,n=21,pg/mL),显著高于非SS患者组(306.8± 120.3,n=21,pg/mL)(t=2.373,P=0.027);唇腺病理阳性组即灶性血清IL-33水平(489.8±170,n=27,pg/mL)高于病理阴性组(1978± 793.1,n=15,pg/mL),2组之间有统计学差异(t=2.368,P=0.023);而抗SSA抗体阳性组与阴性组之间无明显差异(P>0.05).免疫组化染色结果提示SS患者唇腺组织上皮细胞IL-33表达相较于非SS患者上升,且血清IL-33水平与唇腺上皮细胞EpCAM的表达呈中等强度正相关(r=0.4915,P=0.0009,95%CI 0.2205-0.692).结论:IL-33是与SS密切相关的炎症因子,IL-33可能通过促进唾液腺上皮细胞EpCAM的表达参与SS发病.Objective:To investigate the role of interleukin 33(IL-33)in the pathogenesis of Sjogren’s syndrome(SS)by regulating the expression of epithelial cell adhesion molecule(EpCAM).Methods:Serum and labial gland tissue samples,as well as clinical data of patients who needed biopsy of labial gland for SS diagnosis were collected.According to the 2016 ACR-EULAR classification criteria of SS,the patients were divided into SS group and non SS group,and the 21 SS patients and 21 non-SS patients were gender and age matched.The serum IL-33 level was detected by Multi-Analyte Flow Assay Kit(Human Inflammation Panel 1).The difference of IL-33 level between SS group and non SS group,anti SSA antibody positive and negative group and labial gland pathology positive and negative group was compared by t test.Immunohistochemical staining of IL-33 was performed on paraffin section of labial gland tissue.The expression of EpCAM in epithelial cells of fresh labial gland tissues was detected by flow cytometry,and the correlation between Ep CAM expression and serum IL-33 level was conducted by pearson correlation analysis.Results:The level of IL-33 in SS group was(1736±590.1,n=21,pg/mL),which was significantly higher than that in non SS group(306.8±120.3 n=21,pg/mL)(t=2.373,P=0.027);The level of IL-33 in the pathological positive group(489.8±170,n=27,pg/mL)was higher than that in the pathological negative group(1978±793.1,pg/mL),(t=2.368,P=0.023);But there was no significant difference between the anti-SSA positive group and negative group(P>0.05).The immunohistochemical staining of IL-33 in labial gland epithelial cells of SS patients was more intense than that of non SS patients.Besides,the serum level of IL-33 was positively correlated with the expression of EpCAM(r=0.4915,P=0.0009,95%CI0.2205-0.692).Conclusions:IL-33 is a kind of inflammatory cytokine closely related to SS.IL-33 might involve in the pathogenesis of SS through promoting the expression of EpCAM in salivary gland epithelial cells.

关 键 词：干燥综合征 白细胞介素33 上皮细胞黏附分子 涎腺上皮细胞 

分 类 号：R593.2[医药卫生—内科学]