稳定同位素示踪代谢组学技术在葡萄糖分解代谢研究中的应用进展  

Application of stable isotope-resolved metabolomics in glucose catabolism

作　　者：武文泽 令狐婷 高耀[1] 赵云昊 韩雨梅[2] 秦雪梅[1] 田俊生[1] WU Wen-ze;LINGHU Ting;GAO Yao;ZHAO Yun-hao;HAN Yu-mei;QIN Xue-mei;TIAN Jun-sheng(Modern Research Center for Traditional Chinese Medicine,Shanxi University,Taiyuan 030006,China;School of Physical Education,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学体育学院,山西太原030006

出　　处：《药学学报》2021年第5期1286-1292,共7页Acta Pharmaceutica Sinica

基　　金：国家“重大新药创制”科技重大专项(2017ZX09301047);国家自然科学基金资助项目(82074147);山西省科技重点研发计划(201903D321210);山西省“1331工程”协同创新中心建设计划。

摘　　要：随着核磁共振、质谱等高灵敏度检测技术的快速发展,稳定同位素示踪代谢组学技术在阐明代谢通路调控机制以及代谢流分析方面应用日益广泛,且取得了一些突破性的进展。本文对稳定同位素示踪代谢组学在葡萄糖分解代谢调控、代谢流分析、关键代谢通路功能阐释等方面的应用展开综述,为该技术的推广应用及科学研究提供参考依据。With the rapid development of high sensitivity detection techniques such as nuclear magnetic resonance and mass spectrometry,stable isotope-resolved metabolomics has been widely used in elucidating the regulatory mechanism of metabolic pathways and metabolic flow analysis,and some breakthroughs have been made.In this paper the application of stable isotope-resolved metabolomics in glucose catabolic regulation,metabolic flow analysis and functional interpretation of key metabolic pathways is reviewed,providing references for the wider use and application of this technology.

关 键 词：稳定同位素示踪代谢组学 葡萄糖分解代谢 代谢通路 代谢流分析 

分 类 号：R917[医药卫生—药物分析学]