经降解酶修饰的分子印迹干凝胶用于多巴胺选择性吸附和降解  

Degrading enzyme modified molecularly imprinted xerogel for selective adsorption and degradation of dopamine

作　　者：李小畅 陈焘熙 李清晨 刘杨佳 叶田田[2] 王淑君[2] LI Xiao-chang;CHEN Tao-xi;LI Qing-chen;LIU Yang-jia;YE Tian-tian;WANG Shu-jun(School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China;School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《药学学报》2021年第5期1470-1477,共8页Acta Pharmaceutica Sinica

基　　金：国家重点项目,军委后勤保障部应用基础研究重点项目(BWS16J007);国家自然科学基金资助项目(81703455,81973494)。

摘　　要：近年来,因使用成瘾药物产生过量多巴胺(DA)引起关注并亟需解决。分子印迹干凝胶聚合物(molecularly imprinted xerogel polymers, MIXPs)由仿生溶胶-凝胶法制备而成,即聚乙烯亚胺、模板分子DA自组装并以非共价氢键与四甲氧基硅烷交联,洗脱模板DA会留下印迹位点。然后将单胺氧化酶进行MIXPs固定化,得到经酶固定化后的分子印迹干凝胶(monoamine oxidase immobilized MIXPs, MAO-MIXPs),通过制备的人工脑脊液在体外模拟脑中多巴胺表达环境,考察其对多巴胺的选择吸附性能和降解性能,并以吸附性能和降解性能为指标,对MAOMIXPs进行处方优化,确定最佳处方为:DA 40 mg、聚乙烯亚胺0.6 mL与单胺氧化酶2.5 mg·g~(-1)。扫描电子显微镜、差示热量扫描仪和傅立叶变换红外光谱对MAO-MIXPs的性状和理化性质进行表征, Brunner-Emmet-Teller多分子层吸附公式(BET)和兰格缪尔吸附公式(Langmuir)模型模拟,其吸附行为倾向于单层吸附。本研究成功制备了可选择性识别、吸附并降解多巴胺的分子印迹干凝胶聚合物。In recent years,the overexpression dopamine(DA)due to the use of addictive drugs has caused concern and urgently needs to be addressed.The method used in our study is known as biomimetic sol-gel synthesis.We undertook the experiment to develop molecularly imprinted xerogel polymers(MIXPs)through template molecules dopamine polymerized with polyethyleneimine(PEI),then self-assembled and crosslinked with tetramethoxysilane(TMOS)in the form of non-covalent hydrogen bonds by using biomimetic sol-gel process,and then eluted template DA will leave a blotting site.Monoamine oxidase immobilized MIXPs(MAO-MIXPs)was obtained by coating monoamine oxidase onto MIXPs.The synthesis optimization of MAO-MIXPs was finally set as the ratio of DA template,PEI and MAO coating(DA 40 mg,PEI 0.6 mL,MAO 2.5 mg·g-1)to achieve highly selective adsorption toward DA in artificial cerebrospinal fluid based on the adsorption performance and degradation performance.The micromorphologies and physical-chemical properties of MAO-MIXPs were characterized by scanning electron microscopy,differential scanning calorimeter and Fourier transform infrared spectroscopy,and then amount of adsorption was calculated with adsorption equation.Simultaneously,the Brunner-Emmet-Teller(BET)and Langmuir model were simulated.It was found that the adsorption behavior tended to be monolayer adsorption.This new molecularly imprinted polymer demonstrated potential dopamine expression regulation for highly selective recognition,adsorption and degradation of dopamine.

关 键 词：分子印迹聚合物 选择性 吸附 多巴胺 单胺氧化酶 溶胶-凝胶法 

分 类 号：R943[医药卫生—药剂学]