Selection of High-quality Sperms by the Nanotechnological Method of Magnetic Activation in Brazilian Cervids  

作　　者：Teresinha Inês Assumpção AndréLuíz Quagliatto Santos JoséMaurício Barbanti Duarte Maurício Foschini Caroline Silva Vieira 

机构地区：[1]Teaching and Research Laboratory in Wild Animals,Faculty of Veterinary Medicine and Animal Science,Federal University of Uberlandia,Uberlandia,MG,Brazil [2]Deer Research and Conservation Centre,Department of Animal Science,Sao Paulo State University,Jaboticabal,SP,Brazil [3]Physics Institute,Federal University of Uberlandia,Uberlandia,MG,Brazil

出　　处：《Journal of Zoological Research》2021年第2期31-37,共7页动物研究（英文）

摘　　要：Cervids show a high degree of abnormalities in their sperm cells.Thus,this study aimed to select high-quality spermatozoa using magnetic-activated sperm sorting(MASS)compared to density gradient centrifugation(DGC)by assessing the post-selection cell quality.Semen from six Mazama deer was collected by electroejaculation after chemical restraint.The semen was analyzed in four samples:Fresh,DGC,SEMgood-non-apoptotic fraction,and SEMpoor-apoptotic fraction.The material was analyzed for motility and vigor(light microscopy),concentration(Neubauer chamber),semen morphology(phase contrast),and supravital staining test(eosin/nigrosine).The DGC method used 20 x 106 cells in 90%and 45%percoll®gradient.The MASS used 10 x 106 cells with 20μl of iron nanoparticles attached to Annexin V and filtration in a magnetic separation column.Both processing methods(DGC and MASS)were effective in producing high-quality sperm samples,with a marked reduction in abnormalities from 41.83±10.25(fresh)to 14.83±3.17(DGC)and 12±3.01(SEMgood),with 80.3%±2.06 livings cells.These findings suggest that this nanotechnological method,using nanoparticles,effectively produces high-quality semen samples in cervids for use in assisted reproduction.

关 键 词：Reproduction ANDROLOGY FERTILITY DEER EJACULATE Sperm cell 

分 类 号：TG1[金属学及工艺—金属学]