高通量测序技术在慢性肾病患者刚地弓形虫感染诊断中的价值  

Next-generation sequencing for the diagnosis of Toxoplasma gondii infection in patients with chronic kidney disease

作　　者：周毓菁[1] 徐晶晶[1] 徐杰[1] 刘冬梅[1] 何军[1] ZHOU Yujing;XU Jingjing;XU Jie;LIU Dongmei;HE Jun(Center of Clinical Laboratory,the First Affiliated Hospital of Soochow University,Soochow 215000,Jiangsu,China)

机构地区：[1]苏州大学附属第一医院临床检测中心,江苏苏州215000

出　　处：《检验医学》2021年第6期596-599,共4页Laboratory Medicine

基　　金：江苏省医学创新团队与领军人才项目(CXTDB2017009);江苏省社会发展重点项目临床前沿技术(BE2019656)。

摘　　要：目的探讨高通量测序[又称下一代测序(NGS)]技术在临床疑难性病原体诊断中的应用价值。方法收集1例慢性肾脏病5期合并腹痛患者的2份腹水和4份腹膜透析液样本,取沉渣进行镜检,并进行常规细菌及真菌培养。采用化学发光法检测血清弓形虫抗体;采用NGS技术检测腹水和腹膜透析液样本中病原体的基因。结果腹水和腹膜透析液样本镜检发现疑似假包囊。常规细菌和真菌培养未见菌落生长,血清弓形虫IgG和IgM抗体均为阴性。通过NGS确证为刚地弓形虫感染。结论NGS技术对临床疑难性病原体感染的诊断具有显著的优势和极大的应用价值。Objective To investigate the application role of next-generation sequencing(NGS)in the diagnosis of difficult pathogen infection.Methods Totally,2 samples of ascites and 4 samples of peritoneal dialysis fluid were collected from a patient with stage 5 chronic kidney disease complicated with abdominal pain.The morphology of sediments was observed by microscopy directly.Peritoneal dialysis fluid and ascites were cultured using routine method.Toxoplasma IgG and IgM antibodies were detected by chemiluminescence immunoassay.NGS was used to detect the pathogenic genes from peritoneal dialysis fluid and ascites samples.Results The suspected Toxoplasma pseudocapsule was found by microscopy.Microbial culture was showed aseptic growth.Toxoplasma IgG and IgM antibodies were negative reaction.NGS showed there was Toxoplasma gondii infection in the peritoneal dialysis fluid.Conclusions NGS has advantages and application prospects in the diagnosis and treatment guidance of difficult pathogen infection.

关 键 词：刚地弓形虫 腹膜透析液 感染 高通量测序 

分 类 号：R446.1[医药卫生—诊断学]