藏药六味大托叶云实散质量标准研究  

Quality Standard of Tibetan Medicine Liuwei Datuoyeyunshi Powder

作　　者：刘婷婷[1] 杨玲霞 罗宇[1] 陈鸣 LIU Tingting;YANG Lingxia;LUO Yu;CHEN Ming(Department of Pharmacy,The First Affiliated Hospital of Xi’an Jiaotong University,Xi'an,Shaanxi,China 710065;Gansu Institute for Drug Control,Lanzhou,Gansu,China730070)

机构地区：[1]西安交通大学第一附属医院药学部,陕西西安710065 [2]甘肃省药品检验研究院,甘肃兰州730079

出　　处：《中国药业》2021年第11期50-52,共3页China Pharmaceuticals

摘　　要：目的完善藏药六味大托叶云实散的质量标准。方法采用薄层色谱法对方中石榴子、红花进行定性鉴别;采用高效液相色谱法测定红花中羟基红花黄色素A的含量,色谱柱为Agilent C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-乙腈-0.7%磷酸溶液(26∶2∶72,V/V/V),流速为1.0 m L/min,检测波长为403 nm,柱温为25℃,进样量为10μL。结果石榴子、红花的薄层色谱清晰,阴性对照无干扰。羟基红花黄色素A进样量在17.92~215.04μg范围内与峰面积线性关系良好(r=0.9992,n=6);平均加样回收率为100.46%,RSD为0.79%(n=6);精密度、稳定性、重复性试验结果的RSD均小于1.0%(n=6)。结论该方法简便,可操作性强,重复性好,专属性强,可为提高藏药六味大托叶云实散的质量标准提供参考。Objective To improve the quality standard of Tibetan medicine Liuwei Datuoyeyunshi Powder.Methods Thin-layer chromatography(TLC)method was used to qualitatively identify Semen Punicae granati and Carthamus tinctorious in Liuwei Datuoyeyunshi Powder.High-performance liquid chromatography(HPLC)method was used to determine the content of hydroxyl safflower yellow pigment A,the chromatographic column was Agilent C18 column(250 mm×4.6 mm,5μm),the mobile phase was methanol-acetonitrile-0.7%phosphate solution(26∶2∶72,V/V/V),the flow was 1.0 m L/min,the detection wavelength was 403 nm,the column temperature was25℃,and the injection volume was 10μL.Results The TLC spectra of Semen Punicae granati and Carthamus tinctorious were clear,and the negative control had no interference.The mass concentration of hydroxyl safflower yellow pigment A showed a good linear relationship with the peak area in the range of 17.92-215.04μg/m L(r=0.9992,n=6).The average recovery of hydroxyl safflower yellow pigment A was 100.46%with RSD of 0.79%(n=6).The RSDs of precision,stability and repeatability tests were less than1.0%(n=6).Conclusion The method is simple,easy to operate,reproducible and specific,which can provide a reference for improving the quality standard of Tibetan medicine Liuwei Datuoyeyunshi Powder.

关 键 词：藏药 六味大托叶云实散 石榴子 红花 羟基红花黄色素A 薄层色谱法 高效液相色谱法 质量标准 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]