基于双通道探针式81°倾斜光纤光栅的甲胎蛋白免疫检测  

Immunodetection of Alpha-Fetoprotein Based on Dual-Channel Probe Type 81°Tilted Fiber Grating

作　　者：吕清明 罗彬彬[1] 杨琪 张杨[1] 吴胜昔[2] 谷志鹏 石胜辉[1] 吴德操 巫涛江 赵明富[1] Lu Qingming;Luo Binbin;Yang Qi;Zhang Yang;Wu Shengxi;Gu Zhipeng;Shi Shenghui;Wu Decao;Wu Taojiang;Zhao Mingfu(Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection,Chongqing University of Technology,Chongqing 400054,China;Chongqing Key Laboratory of Medicinal Chemistry and Molecular Pharmacology,Chongqing University of Technology,Chongqing 400054,China;Chongqing Energy College,Chongqing 402247,China)

机构地区：[1]重庆理工大学光纤传感与光电检测重庆市重点实验室,重庆400054 [2]重庆理工大学药物化学与分子药理学重庆市重点实验室,重庆400054 [3]重庆能源职业学院,重庆402247

出　　处：《光学学报》2021年第7期29-38,共10页Acta Optica Sinica

基　　金：国家自然科学基金(61875026);重庆市教委科技项目(KJZD-K201901101,KJQN20181121);重庆市自然科学基金(cstc2019jcyj-msxmX0093);重庆理工大学研究生创新项目(ycx20192049,clgycx20202039)。

摘　　要：提出了一种双通道探针式81°倾斜光纤光栅(81°TFG)传感器,并将其用于甲胎蛋白(AFP)的免疫检测研究。首先,在81°TFG端面镀银膜形成反射式81°TFG,构建由2×2耦合器和两支81°TFG探针组成的双通道传感器。然后,在栅区表面修饰大尺寸AuNs粒子和GO,并以AFP单克隆抗体为特异性识别单元修饰栅区表面,实现基于双通道探针式81°TFG局域表面等离子体共振(LSPR)的AFP免疫传感器。最后,在复杂血清环境下完成免疫检测实验,结果表明,该传感器对AFP抗原质量浓度的检测极限在1~10 pg/mL之间,检测的饱和点约为200 ng/mL,灵敏度约为0.155 nm/log(pg/mL),对肝癌患者的血清具有良好的特异性响应。相比单通道透射式81°TFG传感器,该传感器能实现对目标分子阳性和阴性样本的同时对照检测,从而增强临床检测应用中的可靠性,且操作更简便、应用潜力更大。In this study,a dual-channel probe type 81°tilted fiber grating(81°TFG)sensor is proposed and used for the evaluation of alpha-fetoprotein(AFP)immunoassays.First,the end face of 81°TFG is plated with a silver film to form the reflective type 81°TFG,and a dual-channel sensor is constructed by a 2×2 coupler and two 81°TFG probes.Then,large-size AuNs and GO are modified on the grating surface,and AFP monoclonal antibodies are used as the specific recognition unit to modify the grating surface to achieve a dual-channel probe type 81°TFG local surface plasmon resonance(LSPR)AFP immunosensor.Finally,the immune detection experiment is completed in a complex serum environment.The results demonstrated that the detection limit of the sensor for the mass concentration of AFP antigen is within the range of 1--10 pg/mL,the detection saturation point and sensitivity are~200 ng/mL and~0.155 nm/log(pg/mL),respectively,it has a satisfactory specific response to the serum of liver cancer patients is observed.Compared with the single-channel transmission type 81°TFG sensor,the sensor could simultaneously control the detection of positive and negative samples of the target molecules;therefore,enhancing the reliability in clinical detection applications,and the operation is simpler and the application potential is greater.

关 键 词：光纤光学 双通道探针式81°倾斜光纤光栅 局域表面等离子体共振 免疫传感器 甲胎蛋白 

分 类 号：TN253[电子电信—物理电子学]