First Isolation and Confirmation of Sterol Based on β-sitosterol Skeleton from the Leaves of Podocarpusnagi Planted in Fujian, Preliminary in vitro Anticancer Activity and the Crystal Structure  

作　　者：YANG Yang YONG Jian-Ping OLAGOKE Zacchaeus Olatunde LU Can-Zhong 杨洋;雍建平;奥拉特德·奥拉戈克·扎克斯;卢灿忠(Fujian Institute of Research on the Structure of Matter,Haixi Institute,Chinese Academy of Sciences,Fuzhou 350002,China;Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai 201210,China;Xiamen Institute of Rare-earth Materials,Haixi Institute,Chinese Academy of Sciences,Xiamen 361021,China;Shanghai Tech University,Shanghai 201210,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]Fujian Institute of Research on the Structure of Matter,Haixi Institute,Chinese Academy of Sciences,Fuzhou 350002,China [2]Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai 201210,China [3]Xiamen Institute of Rare-earth Materials,Haixi Institute,Chinese Academy of Sciences,Xiamen 361021,China [4]Shanghai Tech University,Shanghai 201210,China [5]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Chinese Journal of Structural Chemistry》2021年第5期653-658,531,共7页结构化学（英文）

基　　金：financially supported by the National Natural Science Foundation of China(21875252);Self-created Area Project of Major Science Technology Innovation Platform of Xiamen(3502ZCQ20171002)。

摘　　要：The crystal of(24 R)-3β,5α-dihydroxy-24-ethyl-5α-cholestan-6-one(1) was isolated from the leaves of P.nagi planted in Fujian for the first time with the same skeleton as 26,27-dinorcholest-5-en-3-β-ol(2) andβ-sitosterol(3) that have been reported before.Compound 1 crystallizes in monoclinic,space group P2_(1) with a=10.8482(4),b=7.3671(3),c=33.7860(15) A,β=93.103(4)°,V=2696.21(19) A^(3),Z=75,M_(r)=446.70,ρ_(calc)=1.145 g/cm^(3),F(000)=1032,μ=0.572 mm^(-1),GOOF=1.034,the final R=0.0467 and wR=0.1129 for 6989 observed reflections with I> 2σ(Ⅰ).Compound 2 was selected to evaluate for their preliminary in vitro anticancer activity against four cancer cell lines for the first time.The results showed that compound 2 exhibited great inhibition against gastric cancer,breast cancer MCF-7,lung cancer A549 and Helacell lines with the inhibitions of89.16% ± 1.17,97.02%±0.53,47.20%±2.58 and 36.89%±1.22 respectively at the concentration of 1.4×10^(-2) M,which means that we found the new anticancer compound in this plant medicine.

关 键 词：Podocarpusnagi crystal structure STEROLS ISOLATION anticancer evaluation 

分 类 号：TQ460.1[化学工程—制药化工]