Peptides-based vaccine against SARS-nCoV-2 antigenic fragmented synthetic epitopes recognized by T cell and β-cell initiation of specific antibodies tofightthe infection  

作　　者：Zainularifeen Abduljaleel Faisal A.Al-Allaf Syed A.Aziz 

机构地区：[1]Department of Medical Genetics,Faculty of Medicine,Umm Al-Qura University,P.O.Box715,Mecca21955,Kingdom of Saudi Arabia [2]Science and Technology Unit,UmmAl-Qura University,P.O.Box 715,Mecca21955,Kingdom of SaudiArabia [3]The Regional Laboratory,Molecular Diagnostics Unit,Department of Molecular Biology,Ministry of Health(MOH),P.O.Box 6251,Mecca,Kingdom of SaudiArabia [4]Department of Pathology and Lab Medicine,University of Ottawa,451Smyth Road,Ottawa,ONK1H8M5,Canada

出　　处：《Bio-Design and Manufacturing》2021年第3期490-505,共16页生物设计与制造（英文）

摘　　要：The World Health Organization has declared the rapidly spreading coronavirus to be a global pandemic.The FDA is yet to approve a vaccine for human novel coronavirus.Here,we developed a peptide-based vaccine and used high-throughput screening by molecular dynamics simulation to identify T-cell-and p-cell-recognized epitopes for producing specific antibod-ies against SARS-nCoV-2.We construct~12 P'antigenic epitope peptides to develop a more effective vaccine and identify specific antibodies.These epitope peptides selectively presented the best antigen presentation scores for both human pMHC class I and II alleles to develop a strong binding affinity.All antigens identified of SARS-nCoV-2 different proteins by each attached specific~1-7 L linker adaptor were used to construct a broad single peripheral peptide vaccine.It is expected to be highly antigenic with a minimum allergic effect.As a result of these exciting outcomes,expressing a vaccine using the intimated peptide was highly promising and positive to be highly proposed as epitope-based peptide vaccine of specific antibody against SARS-nCoV-2 by initiating T cells and β-cells.An in vitro study for the proposed peptide-based vaccine is.mostly recommended.Further clinical trials are required to check the efficacy of this vaccine.

关 键 词：COVID-19 SARS-nCoV-2 Peptide-based vaccine Cell-penetrating peptides Lipid membrane Vaccine efficacy 

分 类 号：R392[医药卫生—免疫学]