气溶胶微生物粒子计数仪光学系统设计  

Design of Optical System of Aerosol Microbial Particle Counter

作　　者：张志强 宋凤民[1] 张秦 党磊 徐溢 李顺波[1] 陈李 Zhang Zhiqiang;Song Fengmin;Zhang Qin;Dang Lei;Xu Yi;Li Shunbo;Chen Li(Key Laboratory of Optoelectronic Technology&System(Chongqing University),Ministry of Education,Key Disciplines Laboratory of Novel Micro-Nano Devices and System Technology,College of Optoelectronic Engineering,Chongqing University,Chongqing 400044,China;State Key Laboratory of Transducer Technology,Chinese Academy of Sciences,Shanghai 200050,China;Aerospace Shenzhou Biotechnology Group Co.,Ltd.,Beijing 100080,China;Beijing Space Bioengineering Technology Research Center,Beijing 100091,China)

机构地区：[1]重庆大学光电工程学院,光电技术与系统教育部重点实验室,新型微纳器件与系统技术重点学科实验室,重庆400044 [2]中国科学院传感技术联合国家重点实验室,上海200050 [3]航天神舟生物科技集团有限公司,北京100080 [4]北京市空间生物工程技术研究中心,北京100091

出　　处：《中国激光》2021年第7期168-175,共8页Chinese Journal of Lasers

基　　金：国家重点研发计划(2018YFB2002302);国家自然科学基金(61971074);民用航天技术预先研究项目(B0107);中国航天科技集团有限公司自主研发项目(201905)。

摘　　要：为了实现微生物粒子在线监测,基于荧光探测原理设计了微生物粒子计数仪光学系统。对微生物粒子含有的核黄素、烟酰胺腺嘌呤二核苷酸等物质的荧光特性进行测试和分析,确定了以405 nm波长的半导体激光器作为激发光源,提出了荧光检测系统的整体结构。设计了基于组合光阑和透镜组的光源整形光路以及荧光探测光路,以降低光噪声。完成了系统结构的制作和组装。采用荧光微球对检测系统的性能进行了测试,结果表明:系统的输出噪声约为20 mV,可以实现对粒径为1,2,5,10μm荧光微球的分级检测,具有信噪比高、检测灵敏度高等特点,对进一步开展气溶胶微生物粒子计数仪的研制具有重要意义。Objective Recently,researchers increasingly focused on air quality.The air microorganisms cause severe harm to human health and severe effects on some industrial production.The latest national standard“Public Places Hygiene Indicators and Limits Requirements”stipulates the total number of air bacteria.Microbial detection based on fluorescence technology is widely used in medicine,pharmaceuticals,food,and environmental monitoring.The detection technologies include real-time fluorescent quantitative polymerase chain reaction(PCR),adenosine triphosphate(ATP)fluorescence detection,and microbial particle fluorescence-sensing system.The fluorescent microbial particle counter can directly count microbial particles,which has some advantages of good real-time performance,a high degree of automation,and simple operation.Besides,it is suitable for real-time online monitoring of the concentration of air microorganisms.This study designs a optical system based on the fluorescence detection principle in microbial particle counter to realize the online monitoring of microbial particles.Methods The optical system is designed based on the fluorescence detection principle.In this study,the fluorescence characteristics of riboflavin,nicotinamide adenine dinucleotide,and other substances contained in microbial particles were tested and analyzed at first.Then,a 405 nm wavelength semiconductor laser was determined as the excitation light source.To reduce the output stray light of the laser,the light source shaping and fluorescence detection optical paths based on the combined diaphragm and lens were designed to obtain a high-quality flat rectangular line spot.Subsequently,consisting of oxidized and blackened aluminum alloy,the optical detection cavity structure were designed in the shape of hexahedron,and the production and assembly of the inspection structure were completed.Finally,the performance of the optical detection system was tested and analyzed using fluorescent microspheres in different particle sizes.Results and Discuss

关 键 词：生物光学 荧光 微生物粒子 光学系统 光噪声 

分 类 号：E933.43[军事—军事装备学] Q631[生物学—生物物理学]