羊BMPR-IB基因非同义单核苷酸多态性的生物信息学分析  

Bioinformatics Analysis of Non-Synonymous Single Nucleotide Polymorphisms of Sheep BMPR-IB Gene

作　　者：冯东青 王慧青 刘月[1] 敦伟涛 刘铮铸[2] 巩元芳[2] 李晓辉[4] 孙洪新 FENG Dongqing;WANG Huiqing;LIU Yue;DUN Weitao;LIU Zhengzhu;GONG Yuanfang;LI Xiaohui;SUN Hongxin(Hebei Institute of Animal Husbandry and Veterinary Medicine,Baoding 071000,China;Hebei Normal University of Science&Technology,Qinghuangdao 066000,China;College of Veterinary Medicine,Hebei Agricultural University,Baoding 071000,China;Agricultural and Rural Bureau of Zhengding County,Zhengding 050800,China)

机构地区：[1]河北省畜牧兽医研究所,保定071000 [2]河北科技师范学院,秦皇岛066000 [3]河北农业大学动物医学院,保定071000 [4]正定县农业农村局,正定050800

出　　处：《中国草食动物科学》2021年第3期1-6,共6页China Herbivore Science

基　　金：河北省羊产业技术体系创新团队保定综合试验推广站建设项目(HBCT2018140403);河北省羊产业技术体系创新团队遗传资源开发与利用岗项目(HBCT2018140201)。

摘　　要：以羊BMPR-IB基因为研究对象,采用生物信息学方法,对该基因潜在的、具有生物学功能的非同义单核苷酸多态性位点(nsSNPs)进行预测,通过SIFI、PolyPhen-2、PROVEN等软件分析预测nsSNPs是否对BMPR-IB蛋白的结构及功能产生影响;通过I-Mutant 3.0、BDM-PUB、GPS-SUMO、Consurf等软件分析预测有害位点nsSNPs对蛋白质稳定性、泛素化修饰位点、磷酸化修饰位点、氨基酸进化位保守水平等是否有影响。结果表明:BMPR-IB基因存在9个nsSNPs位点,其中Q305R、R469K两个位点为主要的有害突变;9个nsSNPs位点BMPR-IB蛋白稳定性均有所降低,其中C35Y、C45S、R44H的稳定性大大降低。预测结果显示,Q305R、R469K为羊BMPR-IB基因的潜在功能性位点。Taking the sheep BMPR-IB gene as the research object,bioinformatics methods were used to predict the potential and biological non-synonymous single nucleotide polymorphisms(nsSNPs)of the gene. It was analyzed and predicted whether nsSNPs would affect the structure and function of BMPR-IB protein by using SIFI,PolyPhen-2,PROVEN and other software.It was also analyzed and predicted whether nsSNPs harmful sites would affect protein stability,ubiquitination modification sites,phosphorylation modification sites,and amino acid evolutionary conservation levels by using software such as I-Mutant 3.0,BDM-PUB,GPS-SUMO,and Consurf.The results showed that 9 nsSNPs sites were screened out,of which 2 sites Q305R and R469K were the main harmful mutations;the stability of BMPR-IB protein at 9 nsSNPs sites was reduced,of which the stability of C35 Y,C45S,and R44H was greatly reduced.In conclusion,that the potential functional sites of sheep BMPR-IB gene were Q305R and R469K.

关 键 词：羊 BMPR-IB基因 非同义单核苷酸多态性 生物信息学分析 

分 类 号：S826.2[农业科学—畜牧学]