超高效液相色谱法测定浓缩饲料中维生素K_(3)含量的不确定度评定  

Uncertainty evaluation of determination of vitamin K_(3) in concentrated feed by UPLC

作　　者：毕融冰 赵卉[1] 王玉方[1] 孙紫薇 金美伶[1] 刘继永[1] Bi Rongbing;Zhao Hui;Wang Yufang;Sun Ziwei;Jin Meiling;Liu Jiyong(Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences,Changchun 130000,China)

机构地区：[1]中国农业科学院特产研究所,长春130000

出　　处：《分析仪器》2021年第3期94-99,共6页Analytical Instrumentation

基　　金：吉林省科技发展计划项目(2019031083NY)资助。

摘　　要：试验采用超高效液相色谱法(UPLC)对浓缩饲料中维生素K_(3)的含量进行测试,并评估此试验的测量不确定度。浓缩饲料中的维生素K_(3)类饲料添加剂经三氯甲烷和碳酸钠溶液提取,转化为游离的甲萘醌,经C_(18)反相色谱柱分离,PDA检测器在251 nm波长处检测,外标法定量即得浓缩饲料中维生素K_(3)的含量。经检测浓缩饲料中维生素K_(3)的含量为:(1.42±0.07)mg·kg^(-1),k=2(95%置信度)。测量不确定度的评定结果表明,标准储备液和标准工作液配制过程是影响测量结果的主要因素。Vitamin K_(3)was extracted by chloroform and sodium carbonate solution and then transformed into free menadione.It was separated by C 18 reversed-phase chromatographic column.The vitamin K_(3)was determined by PDA detector at the wavelength of 251 nm,and quantified by external standard method.The test results showed that the content of vitamin K_(3)was(1.42±0.07)mg·kg^(-1),k=2(95%confidence).The evaluation results showed that the main influence factors were the preparation process of standard stock solution and working solution.

关 键 词：超高效液相色谱法(UPLC) 维生素K_(3) 不确定度评定 

分 类 号：S816.7[农业科学—饲料科学] O657.72[农业科学—畜牧学]