原子力显微镜观察大豆异黄酮类小分子纳米形貌及与血小板的相互作用  

Effect of soybean isoflavone Nano-morphology on platelet morphology and function observed by AFM

作　　者：温晶 阿依江 王耀楠 王玉记[2] 赵淑锐 Wen Jing;A Yijiang;Wang Yaonan;Wang Yuji;Zhao Shurui(Center for Food and Drug Inspection of NMPA,Beijing 100037,China;College of Pharmaceutical Sciences,Capital Medical University,Beijing 100069,China;Core Facilities Center,Capital Medical University,Beijing 100069,China)

机构地区：[1]国家药品监督管理局食品药品审核查验中心,北京100037 [2]首都医科大学药学院,北京100069 [3]首都医科大学中心实验室,北京100069

出　　处：《分析仪器》2021年第3期143-147,共5页Analytical Instrumentation

摘　　要：通过原子力显微镜观察水溶液中分散的大豆异黄酮类小分子(三羟基异黄酮-Lys-Lys-PD)形貌,及其与未活化、活化的血小板相互作用后血小板的形貌变化。结果:三羟基异黄酮-Lys-Lys-PD在水溶液中呈均匀颗粒分布,且颗粒分布比较均一,与未血小板作用后,其在血小板表面的形貌依然可以通过AFM考察,且能够明显观察到其与活化的血小板作用后,血小板的伪足与聚集状态明显减少。The morphology of small soybean isoflavone molecules(Trihydroxyisoflavone-Lys-Lys-PD)dispersed in aqueous solution and the morphology changes of platelets after interaction with inactive and activated platelets were observed by atomic force microscope.The results showed that the particle distribution of Trihydroxyisoflavone-Lys-Lys-PD in the aqueous solution was relatively uniform.After the interaction of Trihydroxyisoflavone-Lys-Lys-PD with inactive platelets,the morphology of the platelet surface can be clearly observed by AFM.The pseudopodia and aggregation state of platelets were significantly reduced by the interaction of Trihydroxyisoflavone-Lys-Lys-PD with activated platelets.

关 键 词：原子力显微镜 血小板形态 大豆异黄酮衍生物 

分 类 号：TQ460.1[化学工程—制药化工]