海口红树根际土壤真菌分离与抗菌活性筛选  

Isolation and antimicrobials activities of mangrove rhizosphere soil-derived fungi from Haikou

作　　者：李敏 朱荣荣 陈芷杞 张启帆 王嘉 沈南星 陈敏 LI Min;ZHU Rongrong;CHEN Zhiqi;ZHANG Qifan;WANG Jia;SHEN Nanxing;CHEN Min(College of Environmental Science and Engineering,Yangzhou University,Yangzhou 225127,China)

机构地区：[1]扬州大学环境科学与工程学院,江苏扬州225127

出　　处：《扬州大学学报（农业与生命科学版）》2021年第2期117-121,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(81703411);江苏省高校大学生创新创业训练计划项目(X20190521)。

摘　　要：红树林生态系统富含抗菌活性真菌资源。对海口市红树根际土壤样品中的真菌进行分离及抗菌活性筛选研究,在分离获得的24株真菌中,菌株Y2-1、J3-2、N4-9的粗提物对耐甲氧西林金黄色葡萄球菌(MRSA)等人类致病菌均具有强抗菌活性,当其浓度为100μg·片^(-1)时抑菌圈达20~30 mm;菌株N4-8的粗提物对水稻纹枯病原菌Rhizoctonia solani的生长具有强抑制作用,当其浓度为100μg·mL^(-1)时抑制率达77.3%。这4株真菌可作为抗MRSA或者抗R.solani的抗菌药物研究的活性菌株资源。Mangrove ecosystem is rich in antimicrobials fungi resources. In this paper, the fungi derived from the mangrove rhizosphere soil samples in Haikou were isolated and their antimicrobials activities were screened. Among the 24 fungi isolates, the extracts of strains Y2-1, J3-2 and N4-9 showed potent antibacterial activity against human pathogenic bacteria such as methicillin-resistant Staphylococcus aureus(MRSA), with an inhibition zone of 20-30 mm at 100 μg·disc^(-1);the extracts of strain N4-8 displayed strong antifungal activity against rice sheath blight pathogen, Rhizoctonia solani, with 77.3% growth inhibition at 100 μg·mL^(-1). These 4 strains can be used as active strain resources for anti-MRSA or anti-R.solani drug research.

关 键 词：红树根际土壤真菌 抗菌活性 耐甲氧西林金黄色葡萄球菌 水稻纹枯病原菌 

分 类 号：S154.39[农业科学—土壤学] R378.11[农业科学—农业基础科学]