电针对高脂饮食诱导的胰岛素抵抗大鼠下丘脑β淀粉样蛋白、Tau蛋白磷酸化水平与糖原合成酶激酶-3的影响  

Effects of Electro-acupuncture on The Amyloid Protein β and Phosphorylation Levels of Tau Protein and GSK-3 in Hypothalamus of Insulin Resistance Rats Induced by High-fat Diet

作　　者：王静芝 杜艳军 陈丽 黄浏娇 瞿涛 周焕娇 陈茜 WANG Jing-zhi;DU Yan-jun;CHEN li;HUANG Liu-jiao;QU Tao;ZHOU Huan-jiao;CHEN Qian(College of Acupuncture Moxibustion and Orthopaedics,Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion,Wuhan 430061,China;Clinical College of Chinese Medicine,Hubei University of Chinese Medicine,Wuhan 430061,China)

机构地区：[1]湖北中医药大学针灸骨伤学院/针灸治未病湖北省协同创新中心,武汉430061 [2]湖北中医药大学中医临床学院,武汉430061

出　　处：《中国中医基础医学杂志》2021年第5期760-764,共5页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金面上项目(81473786)-基于针灸抗炎作用防治阿尔茨海默病星形胶质细胞介导的β-淀粉样蛋白代谢机制研究;国家自然科学基金青年基金项目(81804170)-电针通过SIRT1/ATG7介导的自噬途径调节肝脏脂质代谢改善胰岛素抵抗的机制研究;国家自然科学基金青年基金项目(81904276)-电针通过mTORC1/S6K1自噬途径改善胰岛素抵抗大鼠认知功能损害的机制研究。

摘　　要：目的:观察电针对IR大鼠下丘脑β淀粉样蛋白(Aβ42)、Tau蛋白磷酸化(p-Tau)水平表达与糖原合成酶激酶-3(GSK-3)蛋白表达的影响。方法:70只Wistar大鼠随机分为正常组、模型组、预防组、电针组、预防+阻滞剂组、电针+阻滞剂组和脑脊液组各10只,电针治疗后ELISA法检测各组空腹血糖(FPG)、胰岛素(FINS)与胰岛素抵抗指数(IRI)水平变化,免疫组织化学检测下丘脑Aβ42、p-Tau阳性表达,Western blot检测GSK-3α和GSK-3β蛋白表达。结果:(1)较模型组大鼠,预防组和电针组大鼠FPG、FIN、IRI降低(P<0.01),下丘脑Aβ42和p-Tau阳性表达减少(P<0.05或P<0.01),下丘脑GSK-3α和GSK-3β蛋白表达降低(P<0.05或P<0.01);(2)较电针组大鼠,预防组大鼠FPG、FIN、IRI降低(P<0.05),下丘脑GSK-3α和GSK-3β蛋白表达降低(P<0.05);(3)较电针+阻滞剂组,预防+阻滞剂组大鼠FPG、FINS与IRI均降低(P<0.01),GSK-3β蛋白表达增加(P<0.05)。结论:电针改善IR大鼠FPG、FINS及IRI水平,其作用机制可能与电针抑制GSK-3α和GSK-3β蛋白表达、降低Aβ42和p-Tau阳性表达相关。Objective:To observe the electro-acupuncture effect on the hypothalamic expression levels of Aβ42,phosphorylated Tau protein,GSK-3αand GSK-3βin insulin resistance model rats.Method:70 Wistar rats were divided into 7 groups,10 rats in each group.Fasting plasma glucose and FINs will detect after the treatment by Elisa method,expression levels of hypothalamic Aβ42,phosphorylated Tau protein will detected via immunohistochemistry method;expression levels of hypothalamic GSK-3αand GSK-3βwill detected via western-blotting.Result:(1)Compared with model group,FPG,FINS,ISI reduced(P<0.01),expression levels of hypothalamic Aβ42,phosphorylated Tau protein decreased(P<0.05 or P<0.01),expression levels of hypothalamic GSK-3αand GSK-3βdecreased(P<0.05 or P<0.01)in pre-EA group and EA group;(2)Compared with EA group,FPG,FINS,ISI reduced(P<0.05),expression levels of hypothalamic GSK-3αand GSK-3βdecreased(P<0.05)in pre-EA group;(3)Compared with electro-acupuncture plus blocker LY294002 group,FPG,FINS,ISI reduced(P<0.05),expression levels of hypothalamic GSK-3βincreased(P<0.05)in pre-EA plus blocker LY294002 group;Conclusion:The mechanism of electro-acupuncture improvement on peripheral blood levels of FPG,FINS and IRI in insulin resistance model rats,is pertaining to the regulation of inhibiting the expression of GSK-3αand GSK-3β,and reduces the positive expression of Aβ42 and p-Tau.

关 键 词：电针治疗 胰岛素抵抗 AΒ42 p-Tau GSK-3α GSK-3Β 

分 类 号：R245.97[医药卫生—针灸推拿学]