石蒜碱诱导人肝癌HepG-2细胞凋亡机制研究  

Study on mechanism of lycorine inducing apoptosis of human liver cancer HepG-2 cells

作　　者：李吉业 张晶 于淼 刘斌[1] 辛国松 LI Ji-ye;ZHANG Jing;YU Miao;LIU Bin;XIN Guo-song(Engineering Research Centre for Medicine,Harbin University of Commerce,Harbin 150076,China;Engineering Research Centre of Natural Anticancer Drugs,Ministry of Education,Harbin 150076,China)

机构地区：[1]哈尔滨商业大学药物工程技术研究中心,哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心,哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2021年第3期259-270,共12页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：哈尔滨商业大学创新人才项目(2019CX37)。

摘　　要：利用MTT试验,检测石蒜碱对人肝癌HepG-2细胞具有增殖抑制作用,并观察细胞凋亡形态,采用流式细胞仪检测肿瘤细胞凋亡率,发现石蒜碱对人肝癌HepG-2肿瘤细胞具有显著的抗肿瘤作用;通过对肿瘤细胞膜上主要组成成分进行检测,发现石蒜碱可以改变HepG-2细胞膜主要组成成分,使细胞膜结构发生变化;通过考察石蒜碱对HepG-2细胞细胞膜结构功能的影响,发现石蒜碱可以破坏肿瘤细胞膜完整性,并且能够降低肿瘤细胞膜封闭度、肿瘤细胞膜流动性以及肿瘤细胞膜阳离子通道活性,肿瘤细胞膜主要组成成分、结构的改变将会改变肿瘤细胞的内环境,细胞的内环境的改变必将影响细胞正常的新陈代谢功能,引发细胞凋亡.This paper used the MTT test to detect the proliferation inhibitory effect of lycorine on human liver cancer HepG-2 cells,and observe the morphology of cell apoptosis.Flow cytometry was used to detect the apoptosis rate of tumor cells,and it was found that lycorine was harmful to humans.Liver cancer HepG-2 tumor cells have a significant anti-tumor effect;and through the detection of the main components of the tumor cell membrane,it was found that lycorine can change the main components of the HepG-2 cell membrane and change the cell membrane structure;the effect on the structure and function of the cell membrane of HepG-2 cells.It was found that lycorine can destroy the integrity of tumor cell membranes,and can reduce tumor cell membrane sealing,tumor cell membrane fluidity and tumor cell membrane cation channel activity,and changes in the main components and structure of tumor cell membranes It will change the internal environment of tumor cells.Changes in the internal environment of cells will definitely affect the normal metabolic function of cells,and these changes will trigger cell apoptosis.

关 键 词：石蒜碱 抗肿瘤 肝癌 细胞膜 凋亡率 细胞凋亡 

分 类 号：R284[医药卫生—中药学]