Optimization of Extraction Process and Anti-ultraviolet Activity of Polysaccharide from Cordyceps militaris  

作　　者：Duofei WANG Mengzhen DING Huijuan ZHANG Liyan LI 

机构地区：[1]Medical School,Huanghe Science and Technology University,Zhengzhou 450063,China

出　　处：《Agricultural Biotechnology》2021年第2期89-92,共4页农业生物技术（英文版）

基　　金：College Students Practice Innovation Training Project in Henan Province(No.201911834019);Henan Provincial Department of Education(No.21B350001).

摘　　要：Extraction process of Cordyceps militaris polysaccharide(PS)was optimized with single factor experiment and orthogonal test.Then,the anti-UVB ability of the polysaccharide was investigated to develop anti-UV sun-screening agent through determining the cell viability after UVB irradiation.The results showed that the optimum were solid-liquid ratio at 1∶40(g/ml)at 80℃ for 4 h,and the extract ratio was 27.4%according to the verification test.The PS showed no cytotoxicity to HaCaT cells and pretreatment with the PS significantly decreased UVB-induced cytotoxicity in a dose-dependent relationship.It suggests that the PS has strong anti-UVB irradiation ability and has a good application prospect in developing sunscreen agent.

关 键 词：Cordyceps militaris POLYSACCHARIDE Optimization of extraction conditions Anti-UV radiation 

分 类 号：TQ658.24[化学工程—精细化工]