PEG模拟干旱胁迫对聚合草叶片生理特性的影响  

PEG Simulating Drought Stress:Effect on Physiological Characteristics of Symphytum officinale Leaves

作　　者：刘永华[1] 李鲜花[1] 崔凡 刘翠英[1] Liu Yonghua;Li Xianhua;Cui Fan;Liu Cuiying(Life and Science College,Yulin University,Yulin 719000,Shaanxi,China)

机构地区：[1]榆林学院生命科学学院,陕西榆林719000

出　　处：《农学学报》2021年第6期25-28,共4页Journal of Agriculture

基　　金：陕西省生态恢复与重建实验室开放项目“榆神府煤矿废弃地生态修复植物种的筛选及优化配置研究”(KFXM-201803);榆林市科技局科技攻关项目“榆林市乡土观赏植物调查及园林绿化应用研究”(2018-cxy-2)。

摘　　要：为研究PEG模拟干旱胁迫环境下聚合草叶片的生理变化,设置不同浓度的PEG溶液(0%、5%、10%、15%、20%、25%)进行处理,对其可溶性糖含量、可溶性蛋白含量、游离脯氨酸含量、过氧化物酶活性、超氧化物歧化酶活性进行测定。结果表明,随着PEG浓度(0%~25%)的增加,可溶性糖含量、可溶性蛋白质含量、POD活性、SOD活性均呈现先上升后下降的趋势。当PEG浓度上升到10%时,POD和SOD活性达到最高值,分别为270.3 U/(g·min)、1167.7 U/g;可溶性糖和可溶性蛋白质含量达到最高值,分别为1.9μg/g、236.2μg/g。游离脯氨酸含量随着PEG浓度的升高表现为先下降后上升的趋势,当浓度为10%时,脯氨酸含量到达最低值20.3μg/g。研究结果表明聚合草具有一定的抗旱性,在轻度干旱环境下通过调节能力可以缓解自身受到的伤害。To study the physiological changes of Symphytum officinale leaves under PEG simulating drought stress,different concentrations of PEG(0%,5%,10%,15%20%,25%)were set up.The soluble sugar content,soluble protein content,free proline content,peroxidase activity and superoxide dismutase activity were measured.The results showed that with the increase of PEG concentrations(0%~25%),the soluble sugar content,the soluble protein content,POD activity and SOD activity increased first and then decreased.When the PEG concentration increased to 10%,POD activity and SOD activity reached the highest value,270.3 U/(g·min)and 1167.7 U/g,respectively;the soluble sugar content and the soluble protein content was up to the maximum,1.9μg/g,236.2μg/g,respectively.The free proline content decreased first and then increased with the increase of PEG concentrations.When the concentration was 10%,the proline content reached the lowest value of 20.3μg/g.It indicates that Symphytum officinale has a certain drought resistance,and it could alleviate the damage suffered through own adjustment ability in mild drought environment.

关 键 词：聚合草 PEG 干旱胁迫 生理特性 抗旱性 

分 类 号：S728.2[农业科学—林木遗传育种]