卵母细胞玻璃化冷冻的临床应用价值  

Clinical application value of oocyte vitrification

作　　者：孙贤 姜宏[1] 刘迎春[2] 倪丰 朱杰[2] SUN Xian;JIANG Hong;LIU Yingchun;NI Feng;ZHU Jie(Reproductive Medical Center,Clinical College of the People′s Liberation Army Affiliated to Anhui Medical University,Hefei 230031,China;不详)

机构地区：[1]安徽医科大学解放军临床学院生殖医学中心,合肥230031 [2]中国人民解放军联勤保障部队第901医院生殖医学中心,合肥230031

出　　处：《实用医学杂志》2021年第11期1423-1427,共5页The Journal of Practical Medicine

基　　金：安徽省科技攻关计划项目(编号:1604a0802095)。

摘　　要：目的探讨卵母细胞玻璃化冷冻技术在辅助生殖技术(ART)中应用的可行性、安全性及临床价值。方法回顾性分析行冻融卵母细胞单精子注射⁃胚胎移植(ICSI⁃ET)113个周期患者的临床资料(冻卵组),并按1∶3比例随机抽取同期行新鲜周期ICSI⁃ET共339个周期作为对照组,分析卵母细胞玻璃化冷冻原因,比较两组受精情况及临床妊娠结局。结果卵母细胞冷冻的原因包括取卵日男方无活精子、男方取精失败、家庭意外及女方获卵数较多(≥25枚)等。冷冻卵母细胞复苏率为85.73%,冻卵组无可移植胚胎周期率显著高于对照组,卵裂率、第3天(D3)优质胚胎率显著低于对照组(P<0.05);两组间受精率、D3可移植胚胎率、种植率、临床妊娠率、活产率差异均无统计学意义(P>0.05)。结论卵母细胞玻璃化冷冻可获得与新鲜卵母细胞相近的临床结局,并可作为取卵日无可用精子的补救措施及女性生育力的保存方法。Objective To investigate the feasibility,safety and clinical application value of the oocyte vitrification technology in assisted reproductive technology(ART).Methods The data of 113 cycles performed oocyte vitrification and intracytoplasmic sperm injection⁃embryo transfer(ICSI⁃ET)were analyzed retrospectively(oocyte freezing group),and 339 fresh ICSI⁃ET cycle performed in same period were selected randomly as the control group according to the ratio of 1∶3.The causes of oocyte vitrification were analyzed,the fertilization and clinical pregnancy outcomes were compared between the two groups.Results The reasons for oocyte vitrification included no viable sperm,failing to ejaculate,family emergency events on the day of oocyte retrieval and the wom⁃an gets more oocytes(≥25 oocytes),etc.The recovery rate of oocytes was 85.73%after thawing.The rate of no available embryo cycle in the oocyte freezing group was significantly higher than that in the control group,while the cleavage rate and day 3(D3)′s high⁃quality embryo rate in the oocyte freezing group were significantly lower than those in the control group(P<0.05).There were no statistical differences in fertilization rate,available embryo rate on the D3,implantation rate,clinical pregnancy rate and the live birth rate between the two groups(P>0.05).Conclusion Vitrification of oocytes could obtain similar clinical outcomes to fresh oocytes.Moreover,vitrification of oocytes could be used as a remedy for no available sperm on the day of oocyte retrieval and a method of preserva⁃tion of female fertility.

关 键 词：卵母细胞玻璃化冷冻 卵胞浆内单精子注射⁃胚胎移植 妊娠结局 生育力保存 

分 类 号：R711.6[医药卫生—妇产科学]