异养硝化-好氧反硝化菌株GNR选育及对猪场废水脱氮实验  

ISOLATION OF A HETEROTROPHIC NITRIFICATION-AEROBIC DENITRIFYING STRAIN GNR AND ITS APPLICATION OF AMMONIA REMOVAL OF SWINE WASTEWATER

作　　者：谭俊杰 李立君 李雪洁[1] 韩天放[1] 胡筱敏[1] 赵鑫[1] TAN Jun-jie;LI Li-jun;LI Xue-jie;HAN Tian-fang;HU Xiao-min;ZHAO Xin(Department of Environmental Engineering,School of Resources and Civil Engineering,Northeastern University,Shenyang 110819,China;School of Environment and Society,Tokyo Institute of Technology,Tokyo 152-8550,Japan;Bureau of Ecology and Environment of Panjin,Panjin 124010,China)

机构地区：[1]东北大学资源与土木工程学院,沈阳110819 [2]东京工业大学环境社会理工学院,东京152-8550 [3]盘锦市生态环境局,盘锦124010

出　　处：《环境工程》2021年第2期21-26,共6页Environmental Engineering

基　　金：水体污染控制与治理科技重大专项(2013ZX07202-010);中央直属高校基本科研业务费项目(N170104021,N182508026)。

摘　　要：从养猪场废水中分离获得1株异养硝化-好氧反硝化菌,编号为GNR,经形态学及系统发育鉴定,确定为不动杆菌属(Acinetobacter)的新菌株。通过条件优化实验获得菌株GNR的异养硝化和好氧反硝化的最佳碳源均为柠檬酸三钠,最佳C/N均为10∶1,最佳初始p H分别为8.0和6.0。在最优条件下,初始NH_(4)^(+)-N和NO_(3)^(-)-N浓度均为50.0 mg/L,30℃、125 r/min培养72 h后,硝化和反硝化效率均达到99.0%。菌株GNR在最适宜条件下,对稀释后的实际养猪场废水的COD和NH_(4)^(+)-N去除量最高可达129,73 mg/(L·g),具有良好的污水脱氮潜在应用潜力。In this study,a heterotrophic nitrification-aerobic denitrifying strain named GNR was isolated from swine wastewater.It was identified as a new strain of genus Acinetobacter through morphological and phylogenetic analysis.The optimal heterotrophic nitrification and aerobic denitrification conditions were investigated as the carbon source of trisodium citrate,C/N ratio of 10∶1,initial p H of 8.0 and 6.0,respectively.The maximum removal efficiencies of ammonia and nitrate nitrogen with initial concentration of 50.0 mg/L both reached over 99.0%by strain GNR,after 30℃,125 r/min shaking cultivation for 72 h.Under the optimal condition,the COD and ammonia removal efficiencies in diluted swine wastewater treatment reached 129,73 mg/(L·g),respectively.The experimental results indicated that strain GNR had good application potential in wastewater denitrification.

关 键 词：生物脱氮 异养硝化 好氧反硝化 ACINETOBACTER 养猪场废水 

分 类 号：X713[环境科学与工程—环境工程] X172