血清miR-182、SP-D水平与脓毒症患者预后的关系  

Relationship between serum miR-182,SP-D levels and the prognosis of patients with sepsis

作　　者：吴梅秋 邱名耀 王海曼 黎晓虹 WU Meiqiu;QIU Mingyao;WANG Haiman;LI Xiaohong(Department of Trauma ICU,the First Affiliated Hospital of Hainan Medical College,Haikou,Hainan 570102,China;Department of Emergency,the First Affiliated Hospital of Hainan Medical College,Haikou,Hainan 570102,China)

机构地区：[1]海南医学院第一附属医院创伤ICU,海南海口570102 [2]海南医学院第一附属医院急诊科,海南海口570102

出　　处：《国际检验医学杂志》2021年第12期1436-1440,共5页International Journal of Laboratory Medicine

基　　金：海南省卫生和计划生育委员会行业科研项目(18A200064)。

摘　　要：目的探讨血清微小RNA-182(miR-182)、肺表面活性蛋白D(SP-D)水平与脓毒症患者预后的关系。方法选取该院收治的脓毒症患者120例为脓毒症组,脓毒症组患者又根据28 d时的预后情况分为死亡组26例,存活组94例。另选取非脓毒症引起的全身炎症反应综合征(SIRS)患者120例为SIRS组及健康体检者120例为对照组。采用实时荧光定量PCR检测受试者miR-182水平,采用酶联免疫吸附试验检测受试者SP-D水平。结果与对照组比较,SIRS组、脓毒症组血清miR-182、SP-D水平均明显升高,差异有统计学意义(P<0.05);与SIRS组比较,脓毒症组血清miR-182、SP-D水平均明显升高,差异有统计学意义(P<0.05)。死亡组急性生理与慢性健康状况Ⅱ(APACHEⅡ)评分、血清降钙素原(PCT)、miR-182、SP-D水平均明显高于存活组,差异有统计学意义(P<0.05)。脓毒症患者血清miR-182、SP-D、PCT水平与APACHEⅡ评分均呈正相关(P<0.05)。miR-182低表达组的28 d累积生存率明显高于miR-182高表达组,SP-D低表达组的28 d累积生存率明显高于SP-D高表达组,差异有统计学意义(P<0.05)。miR-182、SP-D、APACHEⅡ评分、PCT水平升高是脓毒症患者死亡的独立危险因素(P<0.05)。结论血清miR-182、SP-D水平升高是脓毒症患者死亡的独立危险因素,可为患者预后评估提供参考。Objective To investigate the relationship between the levels of serum microRNA-182(miR-182),pulmonary surfactant protein D(SP-D)and the prognosis of patients with sepsis.Methods A total of 120 patients with sepsis in the hospital were selected as sepsis group.According to the prognosis at 28 day,the patients in sepsis group were divided into death group(26 cases)and survival group(94 cases).In addition,120 patients with systemic inflammatory response syndrome(SIRS)not caused by sepsis were selected as SIRS group and 120 healthy people took physical examination as control group.Real-time fluorescent quantitative PCR was used to detect the level of miR-182 in subjects,and the level of SP-D in subjects was detected by enzyme-linked immunosorbent assay.Results Compared with control group,the levels of serum miR-182 and SP-D in SIRS group and sepsis group were significantly increased,and the difference was statistically significant(P<0.05).Compared with SIRS group,the levels of serum miR-182 and SP-D in sepsis group were significantly increased,and the difference was statistically significant(P<0.05).The acute physiology and chronic health evaluationⅡ(APACHEⅡ)score,serum procalcitonin(PCT),miR-182 and SP-D levels in death group were significantly higher than those in survival group,and the difference was statistically significant(P<0.05).Serum miR-182,SP-D,PCT levels in patients with sepsis were positively correlated with APACHEⅡscore(P<0.05).The 28 day cumulative survival rate of miR-182 low expression group was significantly higher than that of miR-182 high expression group,and the 28 day cumulative survival rate of SP-D low expression group was significantly higher than that of SP-D high expression group,and the difference was statistically significant(P<0.05).MiR-182,SP-D,APACHEⅡscore and PCT level increased were the independent risk factors of death in patients with sepsis(P<0.05).Conclusion The increase of serum miR-182 and SP-D levels are independent risk factors of death in patients with sepsis,which c

关 键 词：脓毒症 微小RNA-182 肺表面活性蛋白D 预后 

分 类 号：R459.7[医药卫生—急诊医学]