活血散结中药复方含药血浆对bFGF干预下兔视网膜色素上皮细胞增殖的影响  

Influence of plasma containing invigorating blood and dissipating masses Chinese medicine compound on proliferation of rabbit retinal pigment epithelium cell by bFGF intervention

作　　者：蔡娅仙 罗萍[1] 彭俊[1,3] 李建超[1,4] 彭抿 吴权龙[1,5] 彭清华 CAI Ya-xian;LUO Ping;PENG Jun;LI Jian-chao;PENG Min;WU Quan-long;PENG Qing-hua(Key Discipline of Chinese Medical Ophthalmoloy,Hunan University of Chinese Medicine,Changsha 410208,China;Xingsha Hospital of Changsha County,Changsha 410100,China;The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;Xi’an Hospital of Traditional Chinese Medicine,Xi’an 710021,China)

机构地区：[1]湖南中医药大学中医眼科学重点学科,长沙410208 [2]长沙县星沙医院,长沙413200 [3]湖南中医药大学第一附属医院,长沙410007 [4]西安市中医医院,西安710021 [5]湖南中医药大学第一附属医院眼科,长沙410007

出　　处：《中华中医药杂志》2021年第5期2526-2529,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81774372);湖南省中医药管理局科研项目(No.201622);湖南省研究生科研创新项目(No.CX2016B377,No.CX2017B432);中医药防治五官科疾病湖南省重点实验室建设项目(No.2017TP1018);长沙市科技计划项目(No.kel704005);中央财政支持地方高校建设项目;国家中医药管理局中医眼科学重点学科建设项目;湖南省中医五官科学重点学科建设项目。

摘　　要：目的:探索活血散结中药复方含药血浆对碱性成纤维细胞生长因子(bFGF)干预下兔视网膜色素上皮(RPE)细胞增殖的影响。方法:选用对数生长期兔RPE细胞,分成空白对照组、正常血浆组、bFGF组、bFGF+BGJ398组、bFGF+含药血浆组5组,CCK法测定各组兔RPE细胞的细胞活力,流式法检测各组兔RPE细胞的凋亡率。结果:与bFGF组比较,bFGF+BGJ398组、bFGF+含药血浆组细胞活力显著抑制(P<0.01);细胞凋亡率显著增加(P<0.01)。结论:活血散结中药复方含药血浆能有效抑制bFGF干预下兔RPE细胞增殖,促进兔RPE细胞的凋亡。Objective:To explore the inhibition effect of invigorating blood and dissipating masses Chinese medicine compound drug-cotaining plasma on rabbit retinal pigment epithelium(RPE)cell proliferation under intervention of basic fibroblast growth factor(bFGF).Methods:Select the rabbit RPE cells in log phase.The experiment objects were divided into the blank control group,the normal plasma group,the bFGF group,the bFGF+BGJ398 group,the bFGF+drug-containing plasma group.CCK method was used to detect the absorbance value(OD)of rabbit RPE cells from different groups.The flow method was adopted to measure the apoptosis rate of rabbit RPE cells in different groups.Results:Compared with bFGF group,the cell activity of bFGF+BGJ398 group and bFGF+drug-containing plasma group were significantly inhabited(P<0.01);The apoptosis rate was increased evidently(P<0.01).Conclusion:Invigorating blood and dissipating masses Chinese medicine compound drug-cotaining plasma can inhibit the proliferation and promote the apoptosis of rabbit RPE cells under bFGF effect.

关 键 词：活血散结中药复方 含药血浆 增殖 视网膜色素上皮细胞 碱性成纤维细胞生长因子 增殖性玻璃体视网膜病变 

分 类 号：R285[医药卫生—中药学]