白术根腐病病原菌的分离鉴定及特性研究  

Isolation,identification and biological characterization of the pathogenic fungus for Atractylodes macrocephala Koidz.

作　　者：黄幸鸽 李美芽[1] 杨蒋舜 宋腾蛟 袁小凤[2] HUANG Xing-ge;LI Mei-ya;YANG Jiang-shun;SONG Teng-jiao;YUAN Xiao-feng(Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学,杭州310053 [2]浙江中医药大学生命科学学院,杭州310053

出　　处：《中华中医药杂志》2021年第5期2908-2911,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81872951)。

摘　　要：目的:分离白术根腐病病原菌,探究温度和pH值对病原菌生长状态的影响。方法:采用组织分离法分离、纯化得到病原菌,致病性试验判定其对白术的致病性。通过分子生物学鉴定病原菌的种属。分别采用不同的温度和p H值探究不同条件对病原菌生长的影响。结果:白术根腐病病原菌为尖孢镰刀菌(Fusarium oxysporum)。在温度超过30℃、p H值为9时尖孢镰刀菌生长缓慢。结论:温度和pH值都会对尖孢镰刀菌的生长产生影响。Objective:Pathogenic fungus of root rot of Atractylodes macrocephala Koidz.was isolated to explore the effects of temperature and pH on its growth status.Methods:Pathogenic fungus of root rot was isolated by tissue separation method.Exploring the pathogenic effect of isolated strain on Atractylodes macrocephala Koidz.through pathogenicity experiments.The isolated strains were identified by molecular biology methods.Effects of different temperatures and pH on the growth of pathogenic fungus were explored.Results:Fusarium oxysporum was pathogenic fungus of Atractylodes macrocephala Koidz..Fusarium oxysporum growed slowly at temperatures over 30℃and pH=9.Conclusion:Both temperature and pH affect the growth of Fusarium oxysporum.

关 键 词：白术 根腐病 尖孢镰刀菌 温度 p H值 分离 鉴定 特征 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]