HPLC-ELSD法同时测定闹羊花药材中4种成分含量  

Simultaneous determination of four compounds in Rhododendron molle G.Don.by HPLC-ELSD method

作　　者：郭小红[1] 刘霞[1] 吴文辉 王耀登[1] 冷静[2] GUO Xiao-hong;LIU Xia;WU Wen-hui;WANG Yao-deng;LENG Jing(Chongqing Traditional Chinese Medicine Hospital,Chongqing 400021,China)

机构地区：[1]重庆市中医院,重庆400021 [2]重庆市中医院药剂科,重庆400021

出　　处：《中华中医药杂志》2021年第5期2922-2925,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：全国中药特色技术传承人才培训项目(No.T2018482800);重庆市自然科学基金面上项目(No.cstc2019jcyj-msxmX0552);重庆市科研院所绩效激励引导专项(No.cstc2018jxjl130011);成都中医药大学“杏林学者”学科人才科研提升计划(No.YYZX20180023)。

摘　　要：目的:建立HPLC-ELSD同时测定闹羊花中闹羊花毒素Ⅲ、闹羊花毒素Ⅱ、金丝桃苷、槲皮苷的含量测定方法。方法:采用HPLC-ELSD色谱法,Waters C18色谱柱(250mm×4.6mm,5μm);流动相为甲醇(A)和水(B)进行梯度洗脱,流速1.0mL/min,柱温35℃,ELSD参数:雾化温度40℃,漂移管温度85℃,气体压力25psi,气体流速1.5SLM。结果:4种成分线性回归方程的相关系数为0.9990~0.9996,平均回收率(n=9)为97.52%~100.41%,RSD为1.38%~2.22%。结论:该方法简便、准确、可靠,可为闹羊花的质量控制提供参考。Objective:To establish a quantitative method for determining the contents of rhodojaponinⅢ,rhodojaponinⅡ,hyperoside and quercitroside in Rhododendron molle G.Don.by HPLC-ELSD method.Methods:The HPLC-ELSD analysis was performed on Waters C18 column(250 mm×4.6 mm,5μm).The mobile phase was composed of methanol(A)and water(B),and gradient elution was carried out.The flow rate was 1.0 mL/min.The column temperature was maintained at 35℃.ELSD detector parameter:atomization temperature was 40℃.Drift tube temperature was 85℃.Gas pressure was 25 psi,and flow rate of carrier gas was 1.5 SLM.Results:The correlation coefficients of the four compounds ranged from 0.9990 to 0.9996.The average recovery rates(n=9)were between 97.52%and 100.41%,and the RSD were between 1.38%and 2.22%.Conclusion:The proposed method is simple,accurate and reliable,thus providing basis for comprehensive quality control of Rhododendron molle G.Don..

关 键 词：闹羊花 蒸发光散射 二萜 黄酮 含量测定 

分 类 号：R284.1[医药卫生—中药学]