南葶苈子与白芥子寒热药性的细胞学方法评价  

Evaluation on hot&cold characteristics of Descurainia sophia(L.)Webb ex Prantl and Sinapis alba L.by cytological method

作　　者：赵文杰 范铭沁 姚方圆 孙艳妮[1] 刘建利[1] 张宁[1] ZHAO Wen-jie;FAN Ming-qin;YAO Fang-yuan;SUN Yan-ni;LIU Jian-li;ZHANG Ning(Key Laboratory of Resource Biology and Biotechnology in Western China,College of Life Science,Northwest University,Xi’an 710069,China)

机构地区：[1]西部资源生物与现代生物技术教育部重点实验室,西北大学生命科学学院,西安710069

出　　处：《中华中医药杂志》2021年第5期2949-2952,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：陕西省自然科学基金项目(No.2020JM-419,No.2014JM4125);陕西省中医药管理局基金项目(No.2019-ZZ-JC044,No.13-JC012);陕西省教育厅基金项目(No.14JK1737)。

摘　　要：目的:用细胞学方法评价南葶苈子与白芥子的寒热药性。方法:用噻唑蓝(MTT)比色法考察南葶苈子与白芥子对HepG2细胞、SMMC7721细胞、HCT116细胞体外增殖的影响,倒置显微镜观察南葶苈子与白芥子对细胞形态的影响,台盼蓝染色法分析南葶苈子与白芥子的细胞毒作用。结果:南葶苈子在2~800μg/mL浓度范围内对3种细胞的生长增殖表现出抑制作用,随着浓度的增大,抑制率增大,判定为寒性;白芥子在2~800μg/mL浓度范围内对3种细胞的生长增殖表现出低浓度促进、高浓度抑制作用,判定为热性。南葶苈子能使细胞密度减小,固缩变圆;白芥子低浓度下使细胞密度增加,细胞结构致密,生长旺盛,高浓度下使细胞密度降低,固缩变圆。南葶苈子与白芥子对于这3种细胞没有细胞毒作用。结论:南葶苈子和白芥子寒热药性细胞学方法评价结果与文献记载一致;细胞学方法可用于南葶苈子与白芥子寒热药性研究。Objective:To evaluate the hot&cold characteristics of Descurainia sophia(L.)Webb ex Prantl and Sinapis alba L.by cellular models.Methods:MTT assay was used to investigate the effect of Descurainia sophia(L.)Webb ex Prantl and Sinapis alba L.on the proliferation of HepG2 cells,SMMC7721 cells and HCT116 cells in vitro.The morphological changes were observed through inverted microscope.Trypan-blue staining was used to analyze the cytotoxicity of Descurainia sophia(L.)Webb ex Prantl and Sinapis alba L.Results:Descurainia sophia(L.)Webb ex Prantl could inhibite the growth and proliferation of the cells in the concentration range of 2~800μg/mL,that means it possess cold characteristics;Sinapis alba L.could promote the growth and proliferation of the cells at low concentration,and inhibite the growth and proliferation at high concentration.The results means that Sinapis alba L.possess hot characteristics.Cells treated with Descurainia sophia(L.)Webb ex Prantl showing decreased the cell density.Sinapis alba L.increased the cell density at high concentration.Descurainia sophia(L.)Webb ex Prantl and Sinapis alba L.had no cytotoxic effect on the cells.Conclusion:The results of hot&cold characteristics of Descurainia sophia(L.)Webb ex Prantl and Sinapis alba L.obtained in this paper are consistent with traditional literature records.It demonstrated that the simple and reliable cytological method could be used to study the hot&cold characteristics of Descurainia sophia(L.)Webb ex Prantl and Sinapis alba L.

关 键 词：南葶苈子 白芥子 MTT法 Hep G2细胞 SMMC7721细胞 HCT116细胞 台盼蓝染色 寒热药性 

分 类 号：R285.1[医药卫生—中药学]