甜瓜SLAF遗传连锁图谱构建和果柄长度主效QTL定位  

Construction of SLAF Genetic Linkage Map and Mapping of Major QTL for Peduncle Length in Melon(Cucumis melo)

作　　者：戴冬洋 范磊 曾双 王岭[1] 盛云燕[1] 李正洲 张帆[1] DAI Dong-Yang;FAN Lei;ZENG Shuang;WANG Ling;SHENG Yun-Yan;LI Zheng-Zhou;ZHANG Fan(College of Horticulture and Landscape Architecture,Heilongjiang Bayi Agricultural Reclamation University,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学园艺园林学院,大庆163319

出　　处：《农业生物技术学报》2021年第4期656-662,共7页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31772330);黑龙江省自然科学基金(C2017054)。

摘　　要：甜瓜(Cucumis melo)长果柄品种有利于规模化生产与机械化采收。本研究利用长果柄甜瓜’1244’和短果柄甜瓜’MS-5’配置杂交组合,构建F2分离群体,利用特异性位点扩增片段测序(specific-locus amplified fragment sequencing,SLAF-seq)技术构建甜瓜遗传图谱,结合SSR分子标记,对果柄长度(fruit peduncle length,fpl)进行QTL定位。构建的甜瓜遗传图谱包含12个连锁群,获得了10595个上图标记,总图距为1383.88 M,标记间平均距离为0.13 cM;甜瓜果柄长度受2对主基因控制,fpl1.1定位在甜瓜1号染色体CMSSR05887和CMSSR05892之间,表型贡献率为12.2%,QTL候选区间位于甜瓜scaffold0006057524~270836;fpl8.1位于8号染色体CMSSR20549和CMSSR20495之间,表型贡献率为13.2%,QTL候选区间位于scaffold0005894748~105498。研究结果为甜瓜分子标记辅助选择育种及甜瓜机械化采收研究提供理论依据。Long peduncle varieties of melon(Cucumis melo)are beneficial to large-scale production and mechanized harvesting.In present study,long peduncle melon’1244’and short peduncle melon’MS-5’were used to make crossing and constructed F2 segregation population,using specific locus amplified fragment sequencing sequencing(SLAF-seq)technology to construct melon genetic map,combing with SSR molecular markers,to detect fruit peduncle length(fpl)QTL locus.The results showed,a genetic map of 12 linked melon populations,with 10595 markers were constructed,the total distance was 1383.88 cM and the average distance between the markers was 0.13 cM;melon peduncle length was controlled by 2 pairs of main genes,fpl1.1 was located on chromosome 1,the phenotypic contribution rate was 12.2%,QTL candidate interval was between 57524 and 270836 in melon scaffold00060,located between CMSSR05887 and CMSSR05892;fpl8.1 was located on chromosome 8,the phenotypic contribution rate was 13.2%,QTL candidate interval was between 94748 and 105498 in melon scaffold00058,located between CMSSR20549 and CMSSR20495.The results provide a theoretical basis for the study of melon molecular marker assisted selection breeding and melon mechanized harvesting.

关 键 词：甜瓜 果柄长度 遗传规律 特异性位点扩增片段测序(SLAF-seq) SSR QTL定位 

分 类 号：S65[农业科学—果树学]