用于代谢气体分析的化学改性微流控肺泡芯片  

Chemical modified alveolus-on-a-chip microfluidic device for metabolites gas analysis

作　　者：魏昕钰 李明虓 张灵倩 赵阳 黄成军[1,2] WEI Xinyu;LI Mingxiao;ZHANG Lingqian;ZHAO Yang;HUANG Chengjun(Health Electronics Research and Development Center,Institute of Microelectronics,Chinese Academy of Sciences,Beijing 100029,China;School of Electronic Information and Technology,University of Chinese Academy of Sciences,Beijing 100029,China)

机构地区：[1]中国科学院微电子研究所健康电子研发中心,北京100029 [2]中国科学院大学电子信息与技术学院,北京100029

出　　处：《传感器与微系统》2021年第6期20-23,共4页Transducer and Microsystem Technologies

基　　金：国家科技重大专项项目(2018ZX09201-011);北京市自然科学基金资助项目(4182072)。

摘　　要：基于微机电系统(MEMS)微纳加工工艺和聚二甲基硅氧烷(PDMS)软刻蚀技术设计制作了一种包含细胞培养腔体与化学改性气体富集区域的多层微流控肺泡芯片,来进行片上三维细胞培养和细胞代谢的羰基气体捕获,并利用质谱技术对捕获羰基气体进行分析。实验结果表明:该芯片实现了肺癌细胞的片上培养,以及痕量代谢气体捕获。芯片结构简单,样品消耗小,为细胞气体代谢分子体外分析提供了一种有效的手段。An integrated microfluidic device, "alveolus-on-a-chip" is designed and fabricated based on micro-electro-mechanical system(MEMS) technology and polydimethylsiloxane(PDMS) soft lithography technology.The device is suitable for cell culture on a chip and trace volatile metabolites capture under a three-dimensional environment.The capture gas is analyzed by mass spectrometric technique.Experimental results show that the microfluidic chip achieves lung cancer cells culture on the chip and trace metabolic gases capture.Compared with other existing methods, the microfluidic chip shows advantages in simpler structure and less sample consuming, which provides an efficient way to analyze on metabolic gases from cells in vitro.

关 键 词：肺癌细胞 肺泡芯片 化学改性 代谢气体分析 

分 类 号：TH79[机械工程—仪器科学与技术] TP212[机械工程—精密仪器及机械]