鼻咽癌患者miR-429、miR-200C表达与预后关系分析  

Analysis of the relationship between miR-429 and miR-200C expression and prognosis in patients with nasopharyngeal carcinoma

作　　者：王中卫[1] 杨林[1] 郭亚[1] 孙斌[1] 王亚利[1] 马秀龙[1] 任宏涛[1] 包兴[1] WANG Zhongwei;YANG Lin;GUO Ya;SUN Bin;WANG Yali;MA Xiulong;REN Hongtao;BAO Xing(Department of Radiation Therapy,The Second Affiliated Hospital of Xi̓'an Jiaotong University,Xi̓'an 710004,Shaanxi,China)

机构地区：[1]西安交通大学第二附属医院放疗科,陕西西安710004

出　　处：《山东大学耳鼻喉眼学报》2021年第3期81-86,共6页Journal of Otolaryngology and Ophthalmology of Shandong University

摘　　要：目的探讨鼻咽癌患者miR-429、miR-200C表达与预后关系。方法选择2016年1月至2017年5月接受治疗的初治鼻咽癌患者80例,对病理穿刺组织样本行qRT-PCR检测对miR-429、miR-200C相抵表达量进行分析,治疗3个月后进行疗效评价。出院后以电话及门诊复诊的方式进行随访,记录患者生存时间。结果不同病理分期、T分期、N分期及临床分期患者miR-429及miR-200C相对表达量差异均具有统计学意义,且均随严重程度的增加表达量下降(P<0.05)。治疗3个月后80例患者总体有效率为100%,其中CR 71例(88.75%),PR 9例(11.25%)。死亡及复发或转移的55例为预后不良组,无瘤生存的18例为预后良好组,预后良好组患者miR-429及miR-200C相对表达量均高于预后不良组(P<0.05)。miR-429相对表达量对鼻咽癌预后不良预测cut-off值为1.36,AUC为0.723,95%CI为0.547~0.898,灵敏度为82.15%,特异度为81.72%;miR-200C相对表达量对鼻咽癌预后不良预测cut-off值为0.53,AUC为0.713,95%CI为0.562~0.865,灵敏度为82.01%,特异度为79.61%。miR-429高表达组(miR-429相对表达量≥1.36)平均生存时间为(39.07±1.59)个月,95%CI:35.95~42.19;miR-429低表达组(miR-429相对表达量<1.36)平均生存时间为(23.57±1.02)个月,95%CI:21.57~25.58(P<0.05)。miR-200C高表达组(miR-200C相对表达量≥0.53)平均生存时间为(38.28±1.71)个月,95%CI:34.94~41.63;miR-200C低表达组(miR-200C相对表达量<0.53)平均生存时间为(23.52±1.07)个月,95%CI:21.42~25.62(P<0.05)。结论不同病理分期、T分期、N分期及临床分期鼻咽癌患者miR-429、miR-200C相对表达量有差异,且随着病情严重程度的升高miR-429、miR-200C表达量下降,miR-429相对表达量<1.36、miR-200C相对表达量<0.53时鼻咽癌患者生存时间下降。Objective To investigate the relationship between the expression of miR-429,miR-200 C and prognosis in patients with nasopharyngeal carcinoma.Methods 80 patients with nasopharyngeal carcinoma who received treatment between January 2016 and May 2017 were selected Tissue puncture samples were subjected to qRT-PCR detection to analyze the offset expression of miR-429 and miR-200 C,and three treatments were performed.The Efficacy will be evaluated in the coming months.After discharge from the hospital,follow-up was conducted by telephone and outpatient follow-up,and the survival time of the patient was recorded.Results The relative expression levels of miR-429 and miR-200 C in patients with different pathological stages,T stages,N stages and clinical stages were statistically significantly different,and the expression levels decreased with the increase of severity(P<0.05).After three months of treatment,the overall effective rate of 80 patients was 100%,of which 71 were CR(88.75%)and 9 were PR(11.25%).Fifty-five patients with death,recurrence or metastasis belonged to the poor prognosis group,and 18 patients with tumor-free survival belonged to the good prognosis group.The relative expression levels of miR-429 and miR-200 C in the good prognosis group were higher than those in the poor prognosis group(P<0.05).The relative expression of miR-429 has a predictive cut-off value of 1.36 for the poor prognosis of nasopharyngeal carcinoma.The AUC was 0.723,95%CI of 0.547 to 0.898,sensitivity of 82.15%,and the specificity was 81.72%;relative expression of miR-200 C The predictive cut-off value of poor prognosis for nasopharyngeal carcinoma was 0.53,the AUC was 0.713,95%CI was 0.562-0.865,the sensitivity was 82.01%,and the specificity was 79.61%.The average survival time of the miR-429 high expression group(miR-429 relative expression level≥1.36)was 39.07±1.59 months,with a 95%CI:of 35.95-42.19;miR-429 in the low expression group(miR-429 relative expression level<1.36).The average survival time was 23.57±1.02 months,with

关 键 词：鼻咽癌 miR-429 MIR-200C 预后 生存分析 

分 类 号：R766.04[医药卫生—耳鼻咽喉科]