蛹虫草虫草素对ANIT诱导胆汁淤积性肝损伤的保护作用  

Protective effects of cordycepin of Cordyceps militaris on ANIT induced cholestatic liver injury

作　　者：杨晶 倪佳良 高越颖 YANG Jing;NI Jia-Liang;GAO Yue-Ying(School of Pharmaceutical Sciences,Jiangnan University,Wuxi,Jiangsu 214122,China)

机构地区：[1]江南大学药学院,江苏无锡214122

出　　处：《菌物学报》2021年第5期1160-1169,共10页Mycosystema

基　　金：国家自然科学基金(81900560);江苏省自然科学基金(BK2018024);中国博士后基金(2020M671348)。

摘　　要：本研究探讨虫草素对α-萘异硫氰酸酯(ANIT)诱导胆汁淤积性肝损伤的改善作用及保护机制。首先建立ANIT诱导胆汁淤积性肝损伤模型,通过检测血生化指标、HE染色观察肝脏组织病理的情况评价虫草素的保肝作用,进一步通过Westernblot和实时定量PCR技术分析胆汁酸合成、分解、转运以及炎症相关通路的变化。结果显示,与模型组相比,虫草素可以有效降低血清中总胆汁酸水平,改善肝功能,明显降低肝脏病理损伤和炎性细胞浸润。同时,虫草素激活胆汁酸核受体FXR,上调胆汁酸转运体NTCP、BSEP表达,缓解肝内的胆汁酸淤积情况。此外,虫草素还可通过调控NF-κB信号通路抑制肝脏IL-6和IL-1β表达。结果提示,虫草素对ANIT诱导的胆汁淤积模型小鼠肝损伤具有保护作用,其机制可能与其减少肝内胆汁淤积和抑制炎症有关。The potential protective effect of cordycepin on cholestatic liver injury and its mechanism were investigated.An animal model of liver injury induced by ANIT in mice was established.The hepatoprotective effect of cordycepin was estimated by detecting the changes of serum biochemical parameters and observing the pathological changes of tissue sections via HE staining.Western blot analysis and real-time PCR were used to evaluated changes of bile acid synthesis,transport and inflammatory factor.Compared with the model experimental group,cordycepin can effectively reduce serum total bile acid level and improve liver function.Cordycepin significantly reduced liver pathological damage and the infiltration of inflammatory cells.Moreover,cordycepin can activated bile acid nuclear recepter(FXR),up-regulated the expression of bile acid transporters(NTCP,BSEP),alleviating the intrahepatic bile acids.In addition,cordycepin inhibits the expression of IL-6 and IL-1βin the liver via the NF-κB signaling pathway.These results indicate that cordycepin shows hepatoprotective effects on ANIT-induced cholestatic liver injury,and the underlying mechanism may be related to reducing the intrahepatic bile acids and suppressing the inflammatory reaction.

关 键 词：虫草素 胆汁淤积性肝损伤 胆汁酸转运体 胆固醇7Α-羟化酶 

分 类 号：R285.5[医药卫生—中药学]