高速逆流色谱法制备灵芝萜烯酮醇  

Preparation of ganodermenonol by high speed countercurrent chromatography

作　　者：彭小芳 田振[4] 韩伟 王晨光 冯娜[1] 张劲松[1] PENG Xiao-Fang;TIAN Zhen;HAN Wei;WANG Chen-Guang;FENG Na;ZHANG Jing-Song(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungi Processing,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture,China,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China;Engineering Research Centre of Pharmaceutical Process Chemistry,Ministry of Education,Shanghai Key Laboratory of New Drug Design,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China;Shanghai Baixin Bio-Tech Co.,Ltd.,Shanghai 201403,China;Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海市农业科学院食用菌研究所国家食用菌工程技术研究中心国家食用菌加工技术研究分中心农业部南方食用菌资源利用重点实验室上海市农业遗传育种重点开放实验室,上海201403 [2]华东理工大学药学院制药工程与过程化学教育部工程研究中心上海市新药设计重点实验室,上海200237 [3]上海百信生物科技有限公司,上海201403 [4]上海海洋大学,上海201306

出　　处：《菌物学报》2021年第5期1190-1199,共10页Mycosystema

基　　金：上海市科技兴农重点攻关项目(沪农科创字(2018)第1-1号)。

摘　　要：本研究建立一种从灵芝子实体提取物中快速制备灵芝萜烯酮醇的方法。以沪农灵芝1号子实体为原料,经乙醇提取、D101大孔树脂富集后,再经一次正相色谱柱层析,获得富含灵芝萜烯酮醇的流分。采用高速逆流色谱法对该流分进行分离,优化分离条件,获得的最佳条件为:溶剂体系为正己烷-乙酸乙酯-甲醇-水(V/V/V/V,12:24:18:9),流速2.0mL/min,转速900r/min,上样量500mg,一次上样可得到纯度为90.9%的灵芝萜烯酮醇52.1mg,得率达到10.4%。该方法具有操作简单、污染小、成本低、得率和纯度高的特点,是规模化制备灵芝萜烯酮醇的一种新方法。A method to rapidly prepare ganodermenonol from the fruiting body extract of Ganoderma lingzhi was established.The fruiting body of G.lingzhi Hunong No.1 was used as raw material.After ethanol extraction,D101 macroporous resin enrichment,and one time normal phase column chromatography,the fraction rich in ganodermenonol was obtained.The fraction was separated by high-speed countercurrent chromatography,and the separation conditions were optimized.The best conditions obtained were:solvent system of hexane-ethyl acetate-methanol-water(V/V/V/V,12:24:18:9),flow rate of 2.0 mL/min,rotation speed of 900 r/min,and loading quantity of 500 mg.The ganodermenonol yield of 52.1 mg per load could be obtained,with purity of 90.9%and yielding rate of 10.4%.This method is of simple operation,low pollution and cost,with obtained product of high yield and purity,and is a new method for large-scale preparation of ganodermenonol.

关 键 词：灵芝 活性三萜 快速制备 

分 类 号：R285[医药卫生—中药学]