卡帕藻对5-氟尿嘧啶所致肠粘膜损伤的保护作用  

Kappaphycus alvarezii Prevents 5-fluorouracil-induced Intestinal Mucosal Injuryin Mice

作　　者：张青[1] 高远 程枭杰 王志广 李春君 林碧音 唐庆娟[1] ZHANG Qing;GAO Yuan;CHENG Xiao-jie;WANG Zhi-guang;LI Chun-jun;LIN Bi-yin;TANG Qing-juan(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China;Institute of Ocean and Earth Sciences,University of Malaya,Kuala Lumpur 50603,Malaysia)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]马来亚大学海洋与地球科学研究所,吉隆坡50603

出　　处：《现代食品科技》2021年第6期20-27,123,共9页Modern Food Science and Technology

基　　金：国家重点研发计划项目(2018YFC0311201);山东省重点研发计划项目(2019GHY112058)。

摘　　要：探讨两种干燥方式下卡帕藻对5-氟尿嘧啶所致小鼠肠粘膜损伤的保护作用。将35只Balb/c小鼠随机分为5组:正常组(N)、模型组(M)、5-氨基水杨酸组(5-ASA)、晒干卡帕藻组(SKA)、晾干卡帕藻组(AKA)。采用腹腔注射5-FU构建肠粘膜损伤小鼠模型,每日记录小鼠体量和摄食量。实验结束后,统计胸腺和脾脏指数,观察空肠组织学,统计绒毛(villus)长度、隐窝(crypt)深度及两者比值(V/C值),使用酶联免疫法检测血清中TNF-α和IL-10的含量。研究显示,各受试物组的体重增长率高于M组(-3.30%、-6.19%、-5.09%>-7.50%),且第三天可显著抑制小鼠体重(16.85、16.83、16.76>15.75 g)和摄食量(2.15、2.18、2.19>1.54 g)的降低。5-ASA组(5.92 mg/10 g b.w.)和AKA组(6.81 mg/10 g b.w.)的胸腺指数略高于M组(5.67 mg/10 g b.w.)。组织学分析表明,与M组相比,各受试物组均可改善空肠组织学变化和显著上调绒毛长度(332.58、334.44、350.75>252.09μm)(p<0.01)。另外,5-ASA组(6.39)和AKA组(6.35)的V/C值远高于M组(5.12)(p<0.05)。炎症因子方面,与M组相比,AKA组可显著降低血清TNF-α含量(470.73<580.69 pg/mL)(p<0.05)和提高IL-10含量(306.11>287.41 pg/mL)。因此,两种干燥方式下的卡帕藻均可改善5-FU诱导的小鼠肠粘膜损伤,其中晾干卡帕藻的效果更好。Herein,the in vivo protective effects of Kappaphycus alvarezii,dried using two different methods,over 5-fluorouracil(5-FU)-induced intestinal mucosal injury were explored.A total of 35 Balb/c mice were randomly divided into five groups:non-treated group(N),placebo treated group(M),treated with 5-aminosalicylic acid(5-ASA)group,treated with sun-dried K.alvarezii(SKA)group,and treated with air-dried K.alvarezii(AKA)group.Intestinal mucosal injury was induced by intraperitoneal injection of 5-FU,and the body weight(bw)and food intake of the mice were recorded daily.After the experiment,the thymus and spleen indexes were calculated.The jejunum histology was evaluated,including the villus length,crypt depth,and their ratio(V/C value).An enzyme-linked immunoassay was performed to detect the serum levels of the tumor necrosis factor(TNF)-αand interleukin-10.Overall,the weight gain rates of all groups were higher than that of the M group(-3.30%,-6.19%,-5.09%>-7.50%).Moreover,the weight and food intake reduction was significantly suppressed by day 3 of treatment(16.85,16.83,16.76>15.75 g,and 2.15,2.18,2.19>1.54 g).The thymus index of the 5-ASA group(5.92 mg/10 g b.w.)and the AKA group(6.81 mg/10 g b.w.)were slightly higher than that of the M group(5.67 mg/10 g b.w.).Histological analysis showed that,compared with the M group,all treated groups had improved jejunum,with significantly longer villus(332.58,334.44,350.75>252.09μm)(p<0.01).Additionally,the V/C values of the 5-ASA(6.39)and AKA(6.35)groups were much higher than that of the M group(5.12)(p<0.05).Compared with the M group,the AKA group has significantly lower serum TNF-α levels(470.73<580.69 pg/mL)(p<0.05)and remarkably higher interleukin-10(306.11>287.41 pg/mL).Altogether,dried K.alvarezii,regardless of the drying methods used,can alleviate the manifestations of 5-FU-induced intestinal mucosal injury in mice.

关 键 词：卡帕藻 5-氟尿嘧啶 干燥方式 肠黏膜屏障 

分 类 号：R285[医药卫生—中药学]