二甲基亚砜和乙醇处理对西洋参不定根表型和人参皂苷含量的影响  

Effects of Dimethyl Sulfoxide and Ethanol on Adventitious Root Growth of Panax quinquefolium and Ginsenosides

作　　者：胡进 焦红红 南铁贵[2] 赵玉洋 周骏辉[2] 袁媛[2] HU Jin;JIAO Hong-hong;NAN Tie-gui;ZHAO Yu-yang;ZHOU Jun-hui;YUAN Yuan(School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]安徽中医药大学药学院,合肥230012 [2]中国中医科学院中药资源中心道地药材国家重点实验室育种基地,北京100700

出　　处：《中国实验方剂学杂志》2021年第12期141-146,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金重大项目(81891013,81891010);国家科技性基础资源调查专项(2018FY100800);中央本级重大增减支项目(2060302);中央级公益性科研院所基本科研业务费专项(ZZ10-008)。

摘　　要：目的:以西洋参不定根表型和人参皂苷含量为评价指标,筛选合适的助溶剂及浓度,以期为利用西洋参不定根受控实验平台及利用非极性分子化合物进行西洋参"优形、优质"形成机制奠定基础。方法:采用不同体积分数的二甲基亚砜(DMSO)及乙醇助溶剂处理西洋参不定根,利用根系全景扫描仪与WinRHIZO Pro 2016图像处理软件,Synbiosis ProtoCol 3影像分析仪与Image J和根系识别插件SmartRoot相结合的方法,测定不同浓度助溶剂处理后的西洋参不定根分支数及平均直径;采用超高效液相色谱-三重四级杆质谱(UPLC-MS/MS)技术测定不同处理后西洋参不定根中人参皂苷Rg1,Rb1及Re含量。结果:与空白组相比,0.1%DMSO和75%乙醇助溶剂处理后西洋参不定根的表型及人参皂苷含量均发生变化,表现为分支数及平均直径均显著性降低,同时0.1%DMSO处理后导致不定根中人参皂苷Rg1含量降低,75%乙醇处理后增加人参皂苷Rg1和Re的含量,而0.01%DMSO助溶剂处理后西洋参不定根表型及人参皂苷含量与对照组比差异无统计学意义。结论:0.01%DMSO助溶剂对西洋参不定根生长无影响,可作为难溶于水的非极性分子化合物的助溶剂,以上结果为后续深入研究西洋参"优形、优质"成因研究奠定了基础。Objective: To screen out the suitable nonpolar molecular cosolvent and concentration with adventitious root phenotype and ginsenoside content in the controlled experiment as the evaluation indexes,so as to lay a solid foundation for exploring the causes for good shape and high quality of Panax quinquefolium.Method: After being treated with different concentrations of dimethyl sulfoxide(DMSO)and ethanol,the adventitious roots were scanned using a panoramic scanner,and the resulting images were used for measuring the branch number and average diameter by WinRHIZO Pro 2016,Synbiosis ProtoCol 3 colony counter,Image J,and SmartRoot. The contents of ginsenosides Rg1,Rb1,and Re were determined by ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS). Result: Compared with the blank control,the 0.1% DMSO and 75% ethanol made the adventitious root phenotype and ginsenoside contents significantly changed. Specifically,the branch number and average diameter were significantly reduced. The ginsenoside Rg1 in the adventitious roots decreased after 0.1% DMSO treatment,whereas the ginsenosides Rg1 and Re increased after 75% ethanol treatment. The adventitious root phenotype and ginsenoside contents in the 0.1% DMSO treatment group were not significantly different from those in the control group. Conclusion:The 0.01% DMSO does not affect the adventitious root growth of P. quinquefolium and is insoluble in water,enabling it to be considered as a suitable nonpolar molecular cosolvent for future research on the genetic causes for the good shape and high quality of P. quinquefolium.

关 键 词：西洋参 不定根 二甲基亚砜 乙醇 人参皂苷 

分 类 号：R284.2[医药卫生—中药学] R289[医药卫生—中医学]