纳米二氧化钛颗粒职业接触人群尿液非靶向代谢组学研究  

A non-targeted metabolomics study on urine of occupational exposure people with titanium dioxide nanoparticles

作　　者：刘晓东 王超 孟祥娟 潘兴富 李珏 牛东升 陈章健 Liu Xiaodong;Wang Chao;Meng Xiangjuan;Pan Xingfu;Li Jue;Niu Dongsheng;Chen Zhangjian(Beijing Institute of Occupational Disease Prevention and Treatment,Beijing 100093,China;Peking University Health Science Center,Beijing 100191,China)

机构地区：[1]北京市职业病防治研究院,100093 [2]北京大学医学部,100191

出　　处：《中华劳动卫生职业病杂志》2021年第5期328-332,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：中国疾病预防控制中心化学污染与健康安全重点实验室开放基金(2019CDCKL02)。

摘　　要：目的基于代谢组学技术探讨纳米二氧化钛颗粒(TiO_(2) NPs)对职业接触人群尿液中代谢产物的影响,探讨TiO_(2) NPs职业接触致机体早期健康效应的机制。方法于2019年10月,选取TiO_(2) NPs职业接触人群为研究对象,其中接触组64人,从事TiO_(2) NPs接触岗位1年以上;对照组62人,为同企业后勤行政人员。收集研究对象班前尿液,利用超高效液相色谱飞行时间质谱联用仪采集尿液的代谢数据,Progenesis QI软件进行数据的预处理和代谢物的鉴定,SIMCA-P软件对数据进行主成分分析并筛选潜在生物标志物,MetaboAnalyst 4.0软件进行代谢通路富集分析。结果接触组工人的尿液代谢轮廓不同于对照组,筛选并鉴定出44个潜在生物标志物,且在3条通路明显富集(P<0.05),分别为D-精氨酸和D-鸟氨酸代谢通路、氮代谢通路以及D-谷氨酰胺和D-谷氨酸代谢通路。结论TiO_(2) NPs职业接触会对人群尿液中代谢物的浓度和代谢通路产生影响,为TiO_(2) NPs职业危害机制的研究和健康风险的监测提供了方向。Objective To investigate the effects of titanium dioxide nanoparticles(TiO_(2) NPs)on urine metabolites in occupationally exposure people based on metabolomics technology,and to explore the mechanism of early health effects of TiO_(2) NPs on occupational exposure.Methods In October 2019,the TiO_(2) NPs occupational exposure population was selected as the research object,of which 64 people were in the exposure group who had been engaged in TiO_(2) NPs exposure positions for more than 1 year;the control group was 62 people,who were logistics administrative staff of the same company.The urine of the research subjects before class was collected,using the ultra-high performance liquid chromatography time-of-flight mass spectrometer to collect the metabolism data of the urine,Progenesis QI software for data preprocessing and metabolite identification,SIMCA-P software for the principal component analysis of the data and potential biomarkers screening,MetaboAnalyst 4.0 software for metabolic pathway enrichment analysis.Results The urine metabolism profile of workers in the exposure group was different from the control group,and 44 potential biomarkers were screened and identified.These potential biomarkers were significantly enriched in three pathways(P<0.05),namely D-arginine and D-ornithine metabolism pathway,nitrogen metabolism pathway and D-glutamine and D-glutamate metabolism pathways.Conclusion The occupational exposure of TiO_(2) NPs can affect the concentration of metabolites in people urine and metabolic pathways,which provides a direction for the study of occupational hazard mechanisms of TiO_(2) NPs and the monitoring of health risks.

关 键 词：职业暴露 代谢产物学 尿 纳米二氧化钛颗粒 超高效液相色谱飞行时间质谱联用 生物标志物 

分 类 号：R13[医药卫生—劳动卫生]